D1-S169A Substitution of Photosystem II Perturbs Water Oxidation

Ipsita Ghosh, Gourab Banerjee, Christopher J. Kim, Krystle Reiss, Victor S. Batista, Richard J. Debus, Gary W Brudvig

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In photosystem II (PSII), photosynthetic water oxidation occurs at the tetramanganese-calcium cluster that cycles through light-induced intermediates (S 0 -S 4 ) to produce oxygen from two substrate waters. The surrounding hydrogen-bonded amino acid residues and waters form channels that facilitate proton transfer and substrate water delivery, thereby ensuring efficient water oxidation. The residue D1-S169 lies in the "narrow" channel and forms hydrogen bonds with the Mn 4 CaO 5 cluster via waters W1 and Wx. To probe the role of the narrow channel in substrate-water binding, we studied the D1-S169A mutation. PSII core complexes isolated from mutant cells exhibit inefficient S-state cycling and delayed oxygen evolution. The S 2 -state multiline EPR spectrum of D1-S169A PSII core complexes differed significantly from that of wild-type, and FTIR difference spectra showed that the mutation strongly perturbs the extensive network of hydrogen bonds that extends at least from D1-Y161 (Y Z ) to D1-D61. These results imply a possible role of D1-S169 in proton egress or substrate water delivery.

Original languageEnglish
Pages (from-to)1379-1387
Number of pages9
JournalBiochemistry
Volume58
Issue number10
DOIs
Publication statusPublished - Mar 12 2019

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Photosystem II Protein Complex
Substitution reactions
Oxidation
Water
Hydrogen
Substrates
Protons
Hydrogen bonds
Oxygen
Aquaporins
Mutation
Photoperiod
Fourier Transform Infrared Spectroscopy
Proton transfer
Paramagnetic resonance
Calcium
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Ghosh, I., Banerjee, G., Kim, C. J., Reiss, K., Batista, V. S., Debus, R. J., & Brudvig, G. W. (2019). D1-S169A Substitution of Photosystem II Perturbs Water Oxidation. Biochemistry, 58(10), 1379-1387. https://doi.org/10.1021/acs.biochem.8b01184

D1-S169A Substitution of Photosystem II Perturbs Water Oxidation. / Ghosh, Ipsita; Banerjee, Gourab; Kim, Christopher J.; Reiss, Krystle; Batista, Victor S.; Debus, Richard J.; Brudvig, Gary W.

In: Biochemistry, Vol. 58, No. 10, 12.03.2019, p. 1379-1387.

Research output: Contribution to journalArticle

Ghosh, I, Banerjee, G, Kim, CJ, Reiss, K, Batista, VS, Debus, RJ & Brudvig, GW 2019, 'D1-S169A Substitution of Photosystem II Perturbs Water Oxidation', Biochemistry, vol. 58, no. 10, pp. 1379-1387. https://doi.org/10.1021/acs.biochem.8b01184
Ghosh I, Banerjee G, Kim CJ, Reiss K, Batista VS, Debus RJ et al. D1-S169A Substitution of Photosystem II Perturbs Water Oxidation. Biochemistry. 2019 Mar 12;58(10):1379-1387. https://doi.org/10.1021/acs.biochem.8b01184
Ghosh, Ipsita ; Banerjee, Gourab ; Kim, Christopher J. ; Reiss, Krystle ; Batista, Victor S. ; Debus, Richard J. ; Brudvig, Gary W. / D1-S169A Substitution of Photosystem II Perturbs Water Oxidation. In: Biochemistry. 2019 ; Vol. 58, No. 10. pp. 1379-1387.
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