Defining the far-red limit of photosystem II in Spinach

Anders Thapper, Fikret Mamedov, Fredrik Mokvist, Leif Hammarström, Stenbjörn Styring

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The far-red limit of photosystem II (PSII) photochemistry was studied in PSII-enriched membranes and PSII core preparations from spinach (Spinacia olerácea) after application of laser flashes between 730 and 820 nm. Light up to 800 nm was found to drive PSII activity in both acceptor side reduction and oxidation of the water-oxidizing CaMn4 cluster. Farred illumination induced enhancement of, and slowed down decay kinetics of, variable fluorescence. Both effects reflect reduction of the acceptor side of PSII. The effects on the donor side of PSII were monitored using electron paramagnetic resonance spectroscopy. Signals from the S2-, S3-, and S0-states could be detected after one, two, and three far-red flashes, respectively, indicating that PSII underwent conventional S-state transitions. Full PSII turnover was demonstrated by far-red flash-induced oxygen release, with oxygen appearing on the third flash. In addition, both the pheophytin anion and the Tyr Z radical were formed by far-red flashes. The efficiency of this far-red photochemistry in PSII decreases with increasing wavelength. The upper limit for detectable photochemistry in PSII on a single flash was determined to be 780 nm. In photoaccumulation experiments, photochemistry was detectable up to 800 nm. Implications for the energetics and energy levels of the charge separated states in PSII are discussed in light of the presented results.

Original languageEnglish
Pages (from-to)2391-2401
Number of pages11
JournalPlant Cell
Volume21
Issue number8
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Photosystem II Protein Complex
Spinacia oleracea
spinach
photosystem II
Photochemistry
photochemistry
Spinacia
Pheophytins
Oxygen
oxygen
electron paramagnetic resonance spectroscopy
Electron Spin Resonance Spectroscopy
Lighting
anions
Anions
lasers
lighting
wavelengths
Spectrum Analysis
Lasers

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Thapper, A., Mamedov, F., Mokvist, F., Hammarström, L., & Styring, S. (2009). Defining the far-red limit of photosystem II in Spinach. Plant Cell, 21(8), 2391-2401. https://doi.org/10.1105/tpc.108.064154

Defining the far-red limit of photosystem II in Spinach. / Thapper, Anders; Mamedov, Fikret; Mokvist, Fredrik; Hammarström, Leif; Styring, Stenbjörn.

In: Plant Cell, Vol. 21, No. 8, 08.2009, p. 2391-2401.

Research output: Contribution to journalArticle

Thapper, A, Mamedov, F, Mokvist, F, Hammarström, L & Styring, S 2009, 'Defining the far-red limit of photosystem II in Spinach', Plant Cell, vol. 21, no. 8, pp. 2391-2401. https://doi.org/10.1105/tpc.108.064154
Thapper, Anders ; Mamedov, Fikret ; Mokvist, Fredrik ; Hammarström, Leif ; Styring, Stenbjörn. / Defining the far-red limit of photosystem II in Spinach. In: Plant Cell. 2009 ; Vol. 21, No. 8. pp. 2391-2401.
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