Depth-resolved multiphoton polarization microscopy by third-harmonic generation

Dan Oron, Eran Tal, Yaron Silberberg

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)


We achieve depth-resolved polarization microscopy by measuring third-harmonic generation induced by a tightly focused circularly polarized beam. In crystals exhibiting strong birefringence this signal is dominated by positively phase-matched third-harmonic generation. This process occurs in only optically anisotropic media, in which the birefringence compensates for the phase mismatch between the fundamental and the third harmonic induced by dispersion. Both the intensity and the polarization of the emitted signal provide information on the local optical anisotropy. We demonstrate the technique by imaging biogenic crystals in sea urchin larval spicules.

Original languageEnglish
Pages (from-to)2315-2317
Number of pages3
JournalOptics Letters
Issue number23
Publication statusPublished - Dec 1 2003

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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