TY - JOUR
T1 - Depth-resolved structural imaging by third-harmonic generation microscopy
AU - Oron, Dan
AU - Yelin, Dvir
AU - Tal, Eran
AU - Raz, Sefi
AU - Fachima, Rachel
AU - Silberberg, Yaron
N1 - Funding Information:
The authors thank Prof. Lia Addadi and Prof. Stephen Weiner for many helpful and stimulating discussions. We would also like to thank Dr. Ronit Yelin for the Xenopus samples, Dr. Jack J.W.A. van Loon for the bone samples, Dr. Shira Silberberg for the drosophila samples and Prof. Fred H. Wilt for the larval spicule samples. Financial support of this research by the Israel Science Foundation and by the German BMBF is gratefully acknowledged.
PY - 2004/7
Y1 - 2004/7
N2 - Third harmonic generation microscopy is shown to be a robust method for obtaining structural information on a variety of biological specimens. Its nature allows depth-resolved imaging of inhomogeneities with virtually no background from surrounding homogeneous media. With an appropriate illumination geometry, third harmonic generation microscopy is shown to be particularly suitable for imaging of biogenic crystals, enabling extraction of the crystal orientation.
AB - Third harmonic generation microscopy is shown to be a robust method for obtaining structural information on a variety of biological specimens. Its nature allows depth-resolved imaging of inhomogeneities with virtually no background from surrounding homogeneous media. With an appropriate illumination geometry, third harmonic generation microscopy is shown to be particularly suitable for imaging of biogenic crystals, enabling extraction of the crystal orientation.
KW - Multiphoton microscopy
KW - Polarization microscopy
KW - Third harmonic generation
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U2 - 10.1016/S1047-8477(03)00125-4
DO - 10.1016/S1047-8477(03)00125-4
M3 - Article
C2 - 15109600
AN - SCOPUS:1942454762
VL - 147
SP - 3
EP - 11
JO - Journal of Structural Biology
JF - Journal of Structural Biology
SN - 1047-8477
IS - 1
ER -