Depth-resolved structural imaging by third-harmonic generation microscopy

Dan Oron, Dvir Yelin, Eran Tal, Sefi Raz, Rachel Fachima, Yaron Silberberg

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Third harmonic generation microscopy is shown to be a robust method for obtaining structural information on a variety of biological specimens. Its nature allows depth-resolved imaging of inhomogeneities with virtually no background from surrounding homogeneous media. With an appropriate illumination geometry, third harmonic generation microscopy is shown to be particularly suitable for imaging of biogenic crystals, enabling extraction of the crystal orientation.

Original languageEnglish
Pages (from-to)3-11
Number of pages9
JournalJournal of Structural Biology
Volume147
Issue number1
DOIs
Publication statusPublished - Jul 2004

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Keywords

  • Multiphoton microscopy
  • Polarization microscopy
  • Third harmonic generation

ASJC Scopus subject areas

  • Structural Biology

Cite this

Depth-resolved structural imaging by third-harmonic generation microscopy. / Oron, Dan; Yelin, Dvir; Tal, Eran; Raz, Sefi; Fachima, Rachel; Silberberg, Yaron.

In: Journal of Structural Biology, Vol. 147, No. 1, 07.2004, p. 3-11.

Research output: Contribution to journalArticle

Oron, Dan ; Yelin, Dvir ; Tal, Eran ; Raz, Sefi ; Fachima, Rachel ; Silberberg, Yaron. / Depth-resolved structural imaging by third-harmonic generation microscopy. In: Journal of Structural Biology. 2004 ; Vol. 147, No. 1. pp. 3-11.
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