Abstract
Third harmonic generation microscopy is shown to be a robust method for obtaining structural information on a variety of biological specimens. Its nature allows depth-resolved imaging of inhomogeneities with virtually no background from surrounding homogeneous media. With an appropriate illumination geometry, third harmonic generation microscopy is shown to be particularly suitable for imaging of biogenic crystals, enabling extraction of the crystal orientation.
| Original language | English |
|---|---|
| Pages (from-to) | 3-11 |
| Number of pages | 9 |
| Journal | Journal of Structural Biology |
| Volume | 147 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 2004 |
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Keywords
- Multiphoton microscopy
- Polarization microscopy
- Third harmonic generation
ASJC Scopus subject areas
- Structural Biology
Cite this
Depth-resolved structural imaging by third-harmonic generation microscopy. / Oron, Dan; Yelin, Dvir; Tal, Eran; Raz, Sefi; Fachima, Rachel; Silberberg, Yaron.
In: Journal of Structural Biology, Vol. 147, No. 1, 07.2004, p. 3-11.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Depth-resolved structural imaging by third-harmonic generation microscopy
AU - Oron, Dan
AU - Yelin, Dvir
AU - Tal, Eran
AU - Raz, Sefi
AU - Fachima, Rachel
AU - Silberberg, Yaron
PY - 2004/7
Y1 - 2004/7
N2 - Third harmonic generation microscopy is shown to be a robust method for obtaining structural information on a variety of biological specimens. Its nature allows depth-resolved imaging of inhomogeneities with virtually no background from surrounding homogeneous media. With an appropriate illumination geometry, third harmonic generation microscopy is shown to be particularly suitable for imaging of biogenic crystals, enabling extraction of the crystal orientation.
AB - Third harmonic generation microscopy is shown to be a robust method for obtaining structural information on a variety of biological specimens. Its nature allows depth-resolved imaging of inhomogeneities with virtually no background from surrounding homogeneous media. With an appropriate illumination geometry, third harmonic generation microscopy is shown to be particularly suitable for imaging of biogenic crystals, enabling extraction of the crystal orientation.
KW - Multiphoton microscopy
KW - Polarization microscopy
KW - Third harmonic generation
UR - http://www.scopus.com/inward/record.url?scp=1942454762&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1942454762&partnerID=8YFLogxK
U2 - 10.1016/S1047-8477(03)00125-4
DO - 10.1016/S1047-8477(03)00125-4
M3 - Article
VL - 147
SP - 3
EP - 11
JO - Journal of Structural Biology
JF - Journal of Structural Biology
SN - 1047-8477
IS - 1
ER -