Depth-resolved structural imaging by third-harmonic generation microscopy

Dan Oron; Dvir Yelin; Eran Tal; Sefi Raz; Rachel Fachima; Yaron Silberberg

doi:10.1016/S1047-8477(03)00125-4

Depth-resolved structural imaging by third-harmonic generation microscopy

Dan Oron, Dvir Yelin, Eran Tal, Sefi Raz, Rachel Fachima, Yaron Silberberg

Weizmann Institute of Science, Israel

Research output: Contribution to journal › Article › peer-review

76 Citations (Scopus)

Abstract

Third harmonic generation microscopy is shown to be a robust method for obtaining structural information on a variety of biological specimens. Its nature allows depth-resolved imaging of inhomogeneities with virtually no background from surrounding homogeneous media. With an appropriate illumination geometry, third harmonic generation microscopy is shown to be particularly suitable for imaging of biogenic crystals, enabling extraction of the crystal orientation.

Original language	English
Pages (from-to)	3-11
Number of pages	9
Journal	Journal of Structural Biology
Volume	147
Issue number	1
DOIs	https://doi.org/10.1016/S1047-8477(03)00125-4
Publication status	Published - Jul 2004

Keywords

Multiphoton microscopy
Polarization microscopy
Third harmonic generation

ASJC Scopus subject areas

Structural Biology

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10.1016/S1047-8477(03)00125-4

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title = "Depth-resolved structural imaging by third-harmonic generation microscopy",

abstract = "Third harmonic generation microscopy is shown to be a robust method for obtaining structural information on a variety of biological specimens. Its nature allows depth-resolved imaging of inhomogeneities with virtually no background from surrounding homogeneous media. With an appropriate illumination geometry, third harmonic generation microscopy is shown to be particularly suitable for imaging of biogenic crystals, enabling extraction of the crystal orientation.",

keywords = "Multiphoton microscopy, Polarization microscopy, Third harmonic generation",

author = "Dan Oron and Dvir Yelin and Eran Tal and Sefi Raz and Rachel Fachima and Yaron Silberberg",

note = "Funding Information: The authors thank Prof. Lia Addadi and Prof. Stephen Weiner for many helpful and stimulating discussions. We would also like to thank Dr. Ronit Yelin for the Xenopus samples, Dr. Jack J.W.A. van Loon for the bone samples, Dr. Shira Silberberg for the drosophila samples and Prof. Fred H. Wilt for the larval spicule samples. Financial support of this research by the Israel Science Foundation and by the German BMBF is gratefully acknowledged.",

year = "2004",

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AU - Oron, Dan

AU - Yelin, Dvir

AU - Tal, Eran

AU - Raz, Sefi

AU - Fachima, Rachel

AU - Silberberg, Yaron

N1 - Funding Information: The authors thank Prof. Lia Addadi and Prof. Stephen Weiner for many helpful and stimulating discussions. We would also like to thank Dr. Ronit Yelin for the Xenopus samples, Dr. Jack J.W.A. van Loon for the bone samples, Dr. Shira Silberberg for the drosophila samples and Prof. Fred H. Wilt for the larval spicule samples. Financial support of this research by the Israel Science Foundation and by the German BMBF is gratefully acknowledged.

PY - 2004/7

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N2 - Third harmonic generation microscopy is shown to be a robust method for obtaining structural information on a variety of biological specimens. Its nature allows depth-resolved imaging of inhomogeneities with virtually no background from surrounding homogeneous media. With an appropriate illumination geometry, third harmonic generation microscopy is shown to be particularly suitable for imaging of biogenic crystals, enabling extraction of the crystal orientation.

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KW - Multiphoton microscopy

KW - Polarization microscopy

KW - Third harmonic generation

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