Designer nanoscale DNA assemblies programmed from the top down

Remi Veneziano, Sakul Ratanalert, Kaiming Zhang, Fei Zhang, Hao Yan, Wah Chiu, Mark Bathe

Research output: Contribution to journalArticle

207 Citations (Scopus)

Abstract

Scaffolded DNA origami is a versatile means of synthesizing complex molecular architectures. However, the approach is limited by the need to forward-design specific Watson-Crick base pairing manually for any given target structure. Here, we report a general, top-down strategy to design nearly arbitrary DNA architectures autonomously based only on target shape. Objects are represented as closed surfaces rendered as polyhedral networks of parallel DNA duplexes, which enables complete DNA scaffold routing with a spanning tree algorithm. The asymmetric polymerase chain reaction is applied to produce stable, monodisperse assemblies with custom scaffold length and sequence that are verified structurally in three dimensions to be high fidelity by singleparticle cryo-electron microscopy. Their long-term stability in serum and low-salt buffer confirms their utility for biological as well as nonbiological applications.

Original languageEnglish
Article number1534
JournalScience
Volume352
Issue number6293
DOIs
Publication statusPublished - Jun 24 2016

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Cite this

Veneziano, R., Ratanalert, S., Zhang, K., Zhang, F., Yan, H., Chiu, W., & Bathe, M. (2016). Designer nanoscale DNA assemblies programmed from the top down. Science, 352(6293), [1534]. https://doi.org/10.1126/science.aaf4388