Diffraction and imaging from a beam of laser-aligned proteins

Resolution limits

J. C H Spence, K. Schmidt, J. S. Wu, G. Hembree, U. Weierstall, B. Doak, Petra Fromme

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The effect of the limited alignment of hydrated molecules is considered in a laser-aligned molecular beam, on diffraction patterns taken from the beam. Simulated patterns for a protein beam are inverted using the Fienup-Gerchberg-Saxton phasing algorithm, and the effect of limited alignment on the resolution of the resulting potential maps is studied. For a typical protein molecule (lysozyme) with anisotropic polarizability, it is found that up to 1 kW of continuous-wave near-infrared laser power (depending on dielectric constant), together with cooling to liquid-nitrogen temperatures, may be needed to produce sufficiently accurate alignment for direct observation of the secondary structure of proteins in the reconstructed potential or charge-density map. For a typical virus (TMV), a 50 W continuous-wave laser is adequate for subnanometre resolution at room temperature. The dependence of resolution on laser power, temperature, molecular size, shape and dielectric constant is analyzed.

Original languageEnglish
Pages (from-to)237-245
Number of pages9
JournalActa Crystallographica Section A: Foundations of Crystallography
Volume61
Issue number2
DOIs
Publication statusPublished - Mar 2005

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Lasers
Diffraction
alignment
proteins
Proteins
Imaging techniques
Permittivity
diffraction
permittivity
Temperature
lasers
Molecules
Molecular beams
Continuous wave lasers
Infrared lasers
lysozyme
continuous wave lasers
viruses
Liquid nitrogen
Muramidase

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Structural Biology

Cite this

Diffraction and imaging from a beam of laser-aligned proteins : Resolution limits. / Spence, J. C H; Schmidt, K.; Wu, J. S.; Hembree, G.; Weierstall, U.; Doak, B.; Fromme, Petra.

In: Acta Crystallographica Section A: Foundations of Crystallography, Vol. 61, No. 2, 03.2005, p. 237-245.

Research output: Contribution to journalArticle

Spence, J. C H ; Schmidt, K. ; Wu, J. S. ; Hembree, G. ; Weierstall, U. ; Doak, B. ; Fromme, Petra. / Diffraction and imaging from a beam of laser-aligned proteins : Resolution limits. In: Acta Crystallographica Section A: Foundations of Crystallography. 2005 ; Vol. 61, No. 2. pp. 237-245.
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