Dimeric gold nanoparticle assemblies as tags for SERS-based cancer detection

A. Swarnapali D S Indrasekara, Bryan J. Paladini, Dominik J. Naczynski, Valentin Starovoytov, Prabhas V. Moghe, Laura Fabris

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Herein, a new class of multifunctional materials combining a clustered nanoparticle-based probe is presented for surface enhanced Raman scattering (SERS)-based microscopy and surface functionalization for tissue targeting. Controlled assembly of spherical gold nanoparticles into dimers (DNP-REP) is engineered using a small, rigid Raman-active dithiolated linking reporter (REP) to yield narrow internanoparticle gaps and to strategically generate the "hot spot" while concurrently placing the reporter within the region of highest SERS enhancement. Peptide functionalized DNP-REP materials are highly stable even upon incubation with living cells and show controlled levels of binding and intracellular endocytosis. To demonstrate the functionality of such probes for disease detection, differentially targeted DNP-REPs are incubated over various time points with cultured human glioblastoma cells. Using human glioblastoma cells, the SERS maps of targeted tumor cells show the markedly enhanced signals of the DNP-REP, compared to conventional confocal fluorescence based approaches, especially at low incubation times. Even with as few as 40 internalized DNP-REP, a relatively intense SERS signal is measured, demonstrating the high signal to noise ratio and inherent biocompatibility of the materials. Thus, these Raman reporter-based nanoparticle cluster probes present a promising and versatile optical imaging tool for fast, reliable, selective, and ultrasensitive tissue targeting and disease detection and screening.

Original languageEnglish
Pages (from-to)1370-1376
Number of pages7
JournalAdvanced healthcare materials
Volume2
Issue number10
DOIs
Publication statusPublished - Oct 2013

Fingerprint

Raman Spectrum Analysis
Gold
Nanoparticles
Raman scattering
Glioblastoma
Neoplasms
Cells
Optical Imaging
Tissue
Signal-To-Noise Ratio
Endocytosis
Microscopy
Biocompatibility
Fluorescence
Dimers
Peptides
Tumors
Signal to noise ratio
Microscopic examination
Screening

Keywords

  • Dimers
  • Gold nanoparticles
  • Imaging
  • SERS
  • Targeting

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Pharmaceutical Science

Cite this

Indrasekara, A. S. D. S., Paladini, B. J., Naczynski, D. J., Starovoytov, V., Moghe, P. V., & Fabris, L. (2013). Dimeric gold nanoparticle assemblies as tags for SERS-based cancer detection. Advanced healthcare materials, 2(10), 1370-1376. https://doi.org/10.1002/adhm.201200370

Dimeric gold nanoparticle assemblies as tags for SERS-based cancer detection. / Indrasekara, A. Swarnapali D S; Paladini, Bryan J.; Naczynski, Dominik J.; Starovoytov, Valentin; Moghe, Prabhas V.; Fabris, Laura.

In: Advanced healthcare materials, Vol. 2, No. 10, 10.2013, p. 1370-1376.

Research output: Contribution to journalArticle

Indrasekara, ASDS, Paladini, BJ, Naczynski, DJ, Starovoytov, V, Moghe, PV & Fabris, L 2013, 'Dimeric gold nanoparticle assemblies as tags for SERS-based cancer detection', Advanced healthcare materials, vol. 2, no. 10, pp. 1370-1376. https://doi.org/10.1002/adhm.201200370
Indrasekara, A. Swarnapali D S ; Paladini, Bryan J. ; Naczynski, Dominik J. ; Starovoytov, Valentin ; Moghe, Prabhas V. ; Fabris, Laura. / Dimeric gold nanoparticle assemblies as tags for SERS-based cancer detection. In: Advanced healthcare materials. 2013 ; Vol. 2, No. 10. pp. 1370-1376.
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