Direct electrochemistry of photosystem I

O. Kievit, G. W. Brudvig

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31 Citations (Scopus)

Abstract

Direct electrochemistry has been developed into a powerful tool for the analysis of soluble proteins and enzymes over the past two decades. In the present study, we have expanded the range of proteins to include a solubilized membrane protein, photosystem I. Taking into account the difficulties caused by the presence of detergent, we have shown it to be possible to obtain an electrochemical signal of the primary electron donor, a chlorophyll dimer called P700, of photosystem I. A reduction potential of E1/2 = 490mV vs. SHE with a peak separation of ΔE = 100mV was found, in good agreement with literature values.

Original languageEnglish
Pages (from-to)139-149
Number of pages11
JournalJournal of Electroanalytical Chemistry
Volume497
Issue number1-2
DOIs
Publication statusPublished - Feb 2 2001

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Keywords

  • Direct electrochemistry
  • Membrane protein
  • Modified gold electrode
  • Photosystem I

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

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