Direct measurement of the dynamics of hole hopping in extended DNA G-tracts. an unbiased random walk

Sarah M Mickley Conron; Arun K. Thazhathveetil; Michael R Wasielewski; Alexander L. Burin; Frederick D. Lewis

doi:10.1021/ja106991f

Direct measurement of the dynamics of hole hopping in extended DNA G-tracts. an unbiased random walk

Sarah M Mickley Conron, Arun K. Thazhathveetil, Michael R Wasielewski, Alexander L. Burin, Frederick D. Lewis

Northwestern University

Research output: Contribution to journal › Article

70 Citations (Scopus)

Abstract

We report the measurement of distance- and temperature-dependent rate constants for charge separation in capped hairpins in which a stilbene hole acceptor and hole donor are separated by A₃G_n diblock polypurine sequences consisting of 3 adenines and 1-19 guanines. The longer diblock systems obey the simplest model for an unbiased random walk, providing a direct measurement of k_hop = 4.3 × 10⁹ s ^-1 for a single reversible G-to-G hole hopping step, somewhat faster than the value of 1.2 × 10⁹ s^-1 calculated for A-tract hole hopping. The temperature dependence for hopping in A ₃G₁₃ provides values of E_act = 2.8 kcal/mol and A = 7 × 10⁹ s^-1, consistent with a weakly activated, conformationally gated process.

Original language	English
Pages (from-to)	14388-14390
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	132
Issue number	41
DOIs	https://doi.org/10.1021/ja106991f
Publication status	Published - Oct 20 2010

Fingerprint

ASJC Scopus subject areas

Chemistry(all)
Catalysis
Biochemistry
Colloid and Surface Chemistry

Cite this

Conron, S. M. M., Thazhathveetil, A. K., Wasielewski, M. R., Burin, A. L., & Lewis, F. D. (2010). Direct measurement of the dynamics of hole hopping in extended DNA G-tracts. an unbiased random walk. Journal of the American Chemical Society, 132(41), 14388-14390. https://doi.org/10.1021/ja106991f

@article{3acaa409b3c94f9e9a7e3ef819c1f98b,

title = "Direct measurement of the dynamics of hole hopping in extended DNA G-tracts. an unbiased random walk",

abstract = "We report the measurement of distance- and temperature-dependent rate constants for charge separation in capped hairpins in which a stilbene hole acceptor and hole donor are separated by A3Gn diblock polypurine sequences consisting of 3 adenines and 1-19 guanines. The longer diblock systems obey the simplest model for an unbiased random walk, providing a direct measurement of khop = 4.3 × 109 s -1 for a single reversible G-to-G hole hopping step, somewhat faster than the value of 1.2 × 109 s-1 calculated for A-tract hole hopping. The temperature dependence for hopping in A 3G13 provides values of Eact = 2.8 kcal/mol and A = 7 × 109 s-1, consistent with a weakly activated, conformationally gated process.",

author = "Conron, {Sarah M Mickley} and Thazhathveetil, {Arun K.} and Wasielewski, {Michael R} and Burin, {Alexander L.} and Lewis, {Frederick D.}",

year = "2010",

month = "10",

day = "20",

doi = "10.1021/ja106991f",

language = "English",

volume = "132",

pages = "14388--14390",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "41",

}

TY - JOUR

T1 - Direct measurement of the dynamics of hole hopping in extended DNA G-tracts. an unbiased random walk

AU - Conron, Sarah M Mickley

AU - Thazhathveetil, Arun K.

AU - Wasielewski, Michael R

AU - Burin, Alexander L.

AU - Lewis, Frederick D.

PY - 2010/10/20

Y1 - 2010/10/20

N2 - We report the measurement of distance- and temperature-dependent rate constants for charge separation in capped hairpins in which a stilbene hole acceptor and hole donor are separated by A3Gn diblock polypurine sequences consisting of 3 adenines and 1-19 guanines. The longer diblock systems obey the simplest model for an unbiased random walk, providing a direct measurement of khop = 4.3 × 109 s -1 for a single reversible G-to-G hole hopping step, somewhat faster than the value of 1.2 × 109 s-1 calculated for A-tract hole hopping. The temperature dependence for hopping in A 3G13 provides values of Eact = 2.8 kcal/mol and A = 7 × 109 s-1, consistent with a weakly activated, conformationally gated process.

AB - We report the measurement of distance- and temperature-dependent rate constants for charge separation in capped hairpins in which a stilbene hole acceptor and hole donor are separated by A3Gn diblock polypurine sequences consisting of 3 adenines and 1-19 guanines. The longer diblock systems obey the simplest model for an unbiased random walk, providing a direct measurement of khop = 4.3 × 109 s -1 for a single reversible G-to-G hole hopping step, somewhat faster than the value of 1.2 × 109 s-1 calculated for A-tract hole hopping. The temperature dependence for hopping in A 3G13 provides values of Eact = 2.8 kcal/mol and A = 7 × 109 s-1, consistent with a weakly activated, conformationally gated process.

UR - http://www.scopus.com/inward/record.url?scp=77958068669&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77958068669&partnerID=8YFLogxK

U2 - 10.1021/ja106991f

DO - 10.1021/ja106991f

M3 - Article

C2 - 20863110

AN - SCOPUS:77958068669

VL - 132

SP - 14388

EP - 14390

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 41

ER -

Access to Document

10.1021/ja106991f