Direct measurement of the dynamics of hole hopping in extended DNA G-tracts. an unbiased random walk

Sarah M Mickley Conron, Arun K. Thazhathveetil, Michael R Wasielewski, Alexander L. Burin, Frederick D. Lewis

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

We report the measurement of distance- and temperature-dependent rate constants for charge separation in capped hairpins in which a stilbene hole acceptor and hole donor are separated by A3Gn diblock polypurine sequences consisting of 3 adenines and 1-19 guanines. The longer diblock systems obey the simplest model for an unbiased random walk, providing a direct measurement of khop = 4.3 × 109 s -1 for a single reversible G-to-G hole hopping step, somewhat faster than the value of 1.2 × 109 s-1 calculated for A-tract hole hopping. The temperature dependence for hopping in A 3G13 provides values of Eact = 2.8 kcal/mol and A = 7 × 109 s-1, consistent with a weakly activated, conformationally gated process.

Original languageEnglish
Pages (from-to)14388-14390
Number of pages3
JournalJournal of the American Chemical Society
Volume132
Issue number41
DOIs
Publication statusPublished - Oct 20 2010

Fingerprint

DNA
Stilbenes
Temperature
Guanine
Adenine
Rate constants

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Direct measurement of the dynamics of hole hopping in extended DNA G-tracts. an unbiased random walk. / Conron, Sarah M Mickley; Thazhathveetil, Arun K.; Wasielewski, Michael R; Burin, Alexander L.; Lewis, Frederick D.

In: Journal of the American Chemical Society, Vol. 132, No. 41, 20.10.2010, p. 14388-14390.

Research output: Contribution to journalArticle

Conron, Sarah M Mickley ; Thazhathveetil, Arun K. ; Wasielewski, Michael R ; Burin, Alexander L. ; Lewis, Frederick D. / Direct measurement of the dynamics of hole hopping in extended DNA G-tracts. an unbiased random walk. In: Journal of the American Chemical Society. 2010 ; Vol. 132, No. 41. pp. 14388-14390.
@article{3acaa409b3c94f9e9a7e3ef819c1f98b,
title = "Direct measurement of the dynamics of hole hopping in extended DNA G-tracts. an unbiased random walk",
abstract = "We report the measurement of distance- and temperature-dependent rate constants for charge separation in capped hairpins in which a stilbene hole acceptor and hole donor are separated by A3Gn diblock polypurine sequences consisting of 3 adenines and 1-19 guanines. The longer diblock systems obey the simplest model for an unbiased random walk, providing a direct measurement of khop = 4.3 × 109 s -1 for a single reversible G-to-G hole hopping step, somewhat faster than the value of 1.2 × 109 s-1 calculated for A-tract hole hopping. The temperature dependence for hopping in A 3G13 provides values of Eact = 2.8 kcal/mol and A = 7 × 109 s-1, consistent with a weakly activated, conformationally gated process.",
author = "Conron, {Sarah M Mickley} and Thazhathveetil, {Arun K.} and Wasielewski, {Michael R} and Burin, {Alexander L.} and Lewis, {Frederick D.}",
year = "2010",
month = "10",
day = "20",
doi = "10.1021/ja106991f",
language = "English",
volume = "132",
pages = "14388--14390",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "41",

}

TY - JOUR

T1 - Direct measurement of the dynamics of hole hopping in extended DNA G-tracts. an unbiased random walk

AU - Conron, Sarah M Mickley

AU - Thazhathveetil, Arun K.

AU - Wasielewski, Michael R

AU - Burin, Alexander L.

AU - Lewis, Frederick D.

PY - 2010/10/20

Y1 - 2010/10/20

N2 - We report the measurement of distance- and temperature-dependent rate constants for charge separation in capped hairpins in which a stilbene hole acceptor and hole donor are separated by A3Gn diblock polypurine sequences consisting of 3 adenines and 1-19 guanines. The longer diblock systems obey the simplest model for an unbiased random walk, providing a direct measurement of khop = 4.3 × 109 s -1 for a single reversible G-to-G hole hopping step, somewhat faster than the value of 1.2 × 109 s-1 calculated for A-tract hole hopping. The temperature dependence for hopping in A 3G13 provides values of Eact = 2.8 kcal/mol and A = 7 × 109 s-1, consistent with a weakly activated, conformationally gated process.

AB - We report the measurement of distance- and temperature-dependent rate constants for charge separation in capped hairpins in which a stilbene hole acceptor and hole donor are separated by A3Gn diblock polypurine sequences consisting of 3 adenines and 1-19 guanines. The longer diblock systems obey the simplest model for an unbiased random walk, providing a direct measurement of khop = 4.3 × 109 s -1 for a single reversible G-to-G hole hopping step, somewhat faster than the value of 1.2 × 109 s-1 calculated for A-tract hole hopping. The temperature dependence for hopping in A 3G13 provides values of Eact = 2.8 kcal/mol and A = 7 × 109 s-1, consistent with a weakly activated, conformationally gated process.

UR - http://www.scopus.com/inward/record.url?scp=77958068669&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77958068669&partnerID=8YFLogxK

U2 - 10.1021/ja106991f

DO - 10.1021/ja106991f

M3 - Article

VL - 132

SP - 14388

EP - 14390

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 41

ER -