Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold

Guoliang Ke, Minghui Liu, Shuoxing Jiang, Xiaodong Qi, Yuhe Renee Yang, Shaun Wootten, Fei Zhang, Zhi Zhu, Yan Liu, Chaoyong James Yang, Hao Yan

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Artificial multi-enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH-MDH and G6pDH-LDH) through the control of NAD+ substrate channeling by specifically shifting NAD+ between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.

Original languageEnglish
JournalAngewandte Chemie - International Edition
DOIs
Publication statusAccepted/In press - 2016

Fingerprint

Scaffolds (biology)
Scaffolds
DNA
Enzymes
Substrates
NAD
Networks (circuits)
Synthetic Biology

Keywords

  • DNA origami
  • Enzymes
  • NADH
  • Self-assembly
  • Substrate channeling

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

Cite this

Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold. / Ke, Guoliang; Liu, Minghui; Jiang, Shuoxing; Qi, Xiaodong; Yang, Yuhe Renee; Wootten, Shaun; Zhang, Fei; Zhu, Zhi; Liu, Yan; Yang, Chaoyong James; Yan, Hao.

In: Angewandte Chemie - International Edition, 2016.

Research output: Contribution to journalArticle

Ke, Guoliang ; Liu, Minghui ; Jiang, Shuoxing ; Qi, Xiaodong ; Yang, Yuhe Renee ; Wootten, Shaun ; Zhang, Fei ; Zhu, Zhi ; Liu, Yan ; Yang, Chaoyong James ; Yan, Hao. / Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold. In: Angewandte Chemie - International Edition. 2016.
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AU - Yang, Yuhe Renee

AU - Wootten, Shaun

AU - Zhang, Fei

AU - Zhu, Zhi

AU - Liu, Yan

AU - Yang, Chaoyong James

AU - Yan, Hao

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