Directional Regulation of Enzyme Pathways through the Control of Substrate Channeling on a DNA Origami Scaffold

Guoliang Ke, Minghui Liu, Shuoxing Jiang, Xiaodong Qi, Yuhe Renee Yang, Shaun Wootten, Fei Zhang, Zhi Zhu, Yan Liu, Chaoyong James Yang, Hao Yan

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Artificial multi-enzyme systems with precise and dynamic control over the enzyme pathway activity are of great significance in bionanotechnology and synthetic biology. Herein, we exploit a spatially addressable DNA nanoplatform for the directional regulation of two enzyme pathways (G6pDH–MDH and G6pDH–LDH) through the control of NAD+substrate channeling by specifically shifting NAD+between the two enzyme pairs. We believe that this concept will be useful for the design of regulatory biological circuits for synthetic biology and biomedicine.

Original languageEnglish
Pages (from-to)7483-7486
Number of pages4
JournalAngewandte Chemie - International Edition
Volume55
Issue number26
DOIs
Publication statusPublished - Jan 1 2016

Keywords

  • DNA origami
  • NADH
  • enzymes
  • self-assembly
  • substrate channeling

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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