Disassembly and degradation of photosystem I in an in vitro system are multievent, metal-dependent processes

J. Nathan Henderson, Jianying Zhang, B. Walter Evans, Kevin Edward Redding

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

An in vitro system was created to study the process of membrane protein degradation by using photosystem I (PS1) as a model membrane protein. Purified chloroplast membranes were incubated at 30 °C in a defined buffer along with various extracts or reagents to reconstitute the disassembly and degradation of PS1, which was monitored by a variety of techniques that probe the integrity of the PS1 complex: photo-biochemical assays, semi-native gel electrophoresis, low temperature fluorescence spectroscopy, and immunoblots using antibodies against different PS1 subunits. During a typical time course, degradation of PS1 appeared to be a multievent process, with disassembly of the complex preceding proteolysis of the subunits. The first change seen was a rapid (2+, with Co2+ and Ca2+ providing somewhat lower activity.

Original languageEnglish
Pages (from-to)39978-39986
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number41
DOIs
Publication statusPublished - Oct 10 2003

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Photosystem I Protein Complex
Proteolysis
Membrane Proteins
Metals
Degradation
Fluorescence Spectrometry
Chloroplasts
Electrophoresis
Buffers
Gels
Fluorescence spectroscopy
Temperature
Membranes
Antibodies
Assays
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry

Cite this

Disassembly and degradation of photosystem I in an in vitro system are multievent, metal-dependent processes. / Henderson, J. Nathan; Zhang, Jianying; Evans, B. Walter; Redding, Kevin Edward.

In: Journal of Biological Chemistry, Vol. 278, No. 41, 10.10.2003, p. 39978-39986.

Research output: Contribution to journalArticle

Henderson, J. Nathan ; Zhang, Jianying ; Evans, B. Walter ; Redding, Kevin Edward. / Disassembly and degradation of photosystem I in an in vitro system are multievent, metal-dependent processes. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 41. pp. 39978-39986.
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