Distance-dependent electron transfer in DNA hairpins

Frederick D. Lewis, Taifeng Wu, Yifan Zhang, Robert L. Letsinger, Scott R. Greenfield, Michael R Wasielewski

Research output: Contribution to journalArticle

527 Citations (Scopus)

Abstract

The distance dependence of photoinduced electron transfer in duplex DNA was determined for a family of synthetic DNA hairpins in which a stilbene dicarboxamide forms a bridge connecting two oligonucleotide arms. Investigation of the fluorescence and transient absorption spectra of these hairpins established that no photoinduced electron transfer occurs for a hairpin that has six deoxyadenosine-deoxythymidine base pairs. However, the introduction of a single deoxyguanosine-deoxycytidine base pair resulted in distance-dependent fluorescence quenching and the formation of the stilbene union radical. Kinetic analysis suggests that duplex DNA is somewhat more effective than proteins as a medium for electron transfer but that it does not function as a molecular wire.

Original languageEnglish
Pages (from-to)673-676
Number of pages4
JournalScience
Volume277
Issue number5326
DOIs
Publication statusPublished - Aug 1 1997

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Stilbenes
Electrons
Base Pairing
DNA
Fluorescence
Deoxycytidine
Deoxyguanosine
Oligonucleotides
Thymidine
Proteins
2'-deoxyadenosine

ASJC Scopus subject areas

  • General

Cite this

Lewis, F. D., Wu, T., Zhang, Y., Letsinger, R. L., Greenfield, S. R., & Wasielewski, M. R. (1997). Distance-dependent electron transfer in DNA hairpins. Science, 277(5326), 673-676. https://doi.org/10.1126/science.277.5326.673

Distance-dependent electron transfer in DNA hairpins. / Lewis, Frederick D.; Wu, Taifeng; Zhang, Yifan; Letsinger, Robert L.; Greenfield, Scott R.; Wasielewski, Michael R.

In: Science, Vol. 277, No. 5326, 01.08.1997, p. 673-676.

Research output: Contribution to journalArticle

Lewis, FD, Wu, T, Zhang, Y, Letsinger, RL, Greenfield, SR & Wasielewski, MR 1997, 'Distance-dependent electron transfer in DNA hairpins', Science, vol. 277, no. 5326, pp. 673-676. https://doi.org/10.1126/science.277.5326.673
Lewis FD, Wu T, Zhang Y, Letsinger RL, Greenfield SR, Wasielewski MR. Distance-dependent electron transfer in DNA hairpins. Science. 1997 Aug 1;277(5326):673-676. https://doi.org/10.1126/science.277.5326.673
Lewis, Frederick D. ; Wu, Taifeng ; Zhang, Yifan ; Letsinger, Robert L. ; Greenfield, Scott R. ; Wasielewski, Michael R. / Distance-dependent electron transfer in DNA hairpins. In: Science. 1997 ; Vol. 277, No. 5326. pp. 673-676.
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