Distance-dependent interactions between gold nanoparticles and fluorescent molecules with DNA as tunable spacers

Rahul Chhabra, Jaswinder Sharma, Haining Wang, Shengli Zou, Su Lin, Hao Yan, Stuart Lindsay, Yan Liu

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Using stoichiometrically controlled 1:1 functionalization of gold nanoparticles with fluorescent dye molecules in which the dye molecule is held away from the particle surface by a rigid DNA spacer allows precise determination of the distance-dependent effect of the metal nanoparticles on fluorescence intensity. Two dyes were studied, Cy3 and Cy5, with two sizes of nanoparticles, 5 and 10nm. The larger the particle, the more quenching of the photoluminescence (PL) intensity, due to increased overlap of the dye's emission spectrum with the Au surface plasmon resonance. Fluorescence is quenched significantly for distances somewhat larger than the particle diameter, in good agreement with the predictions of an electrodynamics model based on interacting dipoles. The distance dependence of surface energy transfer behavior, i.e.quenching efficiency, is proportional to 1/d4, which involves no consideration of the size of the particle and the spectral overlap of the dye and AuNp. This surface energy transfer model is found qualitatively and agrees with the electrodynamic model, though the exponent is greater than 4 for the smaller nanoparticles (5nm), and smaller than 4 for the larger nanoparticles (10nm).

Original languageEnglish
Article number485201
JournalNanotechnology
Volume20
Issue number48
DOIs
Publication statusPublished - 2009

Fingerprint

Gold
DNA
Coloring Agents
Dyes
Nanoparticles
Molecules
Electrodynamics
Interfacial energy
Energy transfer
Quenching
Fluorescence
Intergenic DNA
Metal nanoparticles
Surface plasmon resonance
Fluorescent Dyes
Photoluminescence

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Cite this

Distance-dependent interactions between gold nanoparticles and fluorescent molecules with DNA as tunable spacers. / Chhabra, Rahul; Sharma, Jaswinder; Wang, Haining; Zou, Shengli; Lin, Su; Yan, Hao; Lindsay, Stuart; Liu, Yan.

In: Nanotechnology, Vol. 20, No. 48, 485201, 2009.

Research output: Contribution to journalArticle

Chhabra, Rahul ; Sharma, Jaswinder ; Wang, Haining ; Zou, Shengli ; Lin, Su ; Yan, Hao ; Lindsay, Stuart ; Liu, Yan. / Distance-dependent interactions between gold nanoparticles and fluorescent molecules with DNA as tunable spacers. In: Nanotechnology. 2009 ; Vol. 20, No. 48.
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AU - Yan, Hao

AU - Lindsay, Stuart

AU - Liu, Yan

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