DNA-cholesterol barges as programmable membrane-exploring agents

Alexander Johnson-Buck, Shuoxing Jiang, Hao Yan, Nils G. Walter

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

DNA nanotechnology enables the precise construction of nanoscale devices that mimic aspects of natural biomolecular systems yet exhibit robustly programmable behavior. While many important biological processes involve dynamic interactions between components associated with phospholipid membranes, little progress has been made toward creating synthetic mimics of such interfacial systems. We report the assembly and characterization of cholesterol-labeled DNA origami barges capable of reversible association with and lateral diffusion on supported lipid bilayers. Using single-particle fluorescence microscopy, we show that these DNA barges rapidly and stably embed in lipid bilayers and exhibit Brownian diffusion in a manner dependent on both cholesterol labeling and bilayer composition. Tracking of individual barges rapidly generates super-resolution maps of the contiguous regions of a membrane. Addition of appropriate command oligonucleotides enables membrane-associated barges to reversibly exchange fluorescent cargo with bulk solution, dissociate from the membrane, or form oligomers within the membrane, opening up new possibilities for programmable membrane-bound molecular devices.

Original languageEnglish
Pages (from-to)5641-5649
Number of pages9
JournalACS Nano
Volume8
Issue number6
DOIs
Publication statusPublished - Jun 24 2014

Fingerprint

Barges
Cholesterol
cholesterol
DNA
deoxyribonucleic acid
membranes
Membranes
Lipid bilayers
Lipid Bilayers
lipids
Biological Phenomena
Equipment and Supplies
Nanotechnology
cargo
oligonucleotides
Oligonucleotides
Fluorescence microscopy
Phospholipids
commands
nanotechnology

Keywords

  • diffusion
  • DNA nanotechnology
  • DNA origami
  • lipid bilayer
  • single-particle tracking

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)
  • Medicine(all)

Cite this

DNA-cholesterol barges as programmable membrane-exploring agents. / Johnson-Buck, Alexander; Jiang, Shuoxing; Yan, Hao; Walter, Nils G.

In: ACS Nano, Vol. 8, No. 6, 24.06.2014, p. 5641-5649.

Research output: Contribution to journalArticle

Johnson-Buck, A, Jiang, S, Yan, H & Walter, NG 2014, 'DNA-cholesterol barges as programmable membrane-exploring agents', ACS Nano, vol. 8, no. 6, pp. 5641-5649. https://doi.org/10.1021/nn500108k
Johnson-Buck, Alexander ; Jiang, Shuoxing ; Yan, Hao ; Walter, Nils G. / DNA-cholesterol barges as programmable membrane-exploring agents. In: ACS Nano. 2014 ; Vol. 8, No. 6. pp. 5641-5649.
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