DNA origami with complex curvatures in three-dimensional space

Dongran Han, Suchetan Pal, Jeanette Nangreave, Zhengtao Deng, Yan Liu, Hao Yan

Research output: Contribution to journalArticle

595 Citations (Scopus)

Abstract

We present a strategy to design and construct self-assembling DNA nanostructures that define intricate curved surfaces in three-dimensional (3D) space using the DNA origami folding technique. Double-helical DNA is bent to follow the rounded contours of the target object, and potential strand crossovers are subsequently identified. Concentric rings of DNA are used to generate in-plane curvature, constrained to 2D by rationally designed geometries and crossover networks. Out-of-plane curvature is introduced by adjusting the particular position and pattern of crossovers between adjacent DNA double helices, whose conformation often deviates from the natural, B-form twist density. A series of DNA nanostructures with high curvature-such as 2D arrangements of concentric rings and 3D spherical shells, ellipsoidal shells, and a nanoflask-were assembled.

Original languageEnglish
Pages (from-to)342-346
Number of pages5
JournalScience
Volume332
Issue number6027
DOIs
Publication statusPublished - Apr 15 2011

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DNA
Nanostructures

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DNA origami with complex curvatures in three-dimensional space. / Han, Dongran; Pal, Suchetan; Nangreave, Jeanette; Deng, Zhengtao; Liu, Yan; Yan, Hao.

In: Science, Vol. 332, No. 6027, 15.04.2011, p. 342-346.

Research output: Contribution to journalArticle

Han, Dongran ; Pal, Suchetan ; Nangreave, Jeanette ; Deng, Zhengtao ; Liu, Yan ; Yan, Hao. / DNA origami with complex curvatures in three-dimensional space. In: Science. 2011 ; Vol. 332, No. 6027. pp. 342-346.
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