DNA-Peptide Amphiphile Nanofibers Enhance Aptamer Function

Christopher M. Serrano, Ronit Freeman, Jacqueline Godbe, Jacob A. Lewis, Samuel I Stupp

Research output: Contribution to journalArticle

Abstract

The single-stranded DNA oligonucleotides known as aptamers have the capacity to bind proteins and other molecules and offer great therapeutic potential. Further work is required to optimize their function and to diminish their susceptibility to nuclease degradation. We report here on the synthesis and supramolecular self-assembly of DNA-peptide amphiphiles that form high aspect ratio nanofibers and display aptamers for the platelet-derived growth factor. The nanofibers were found to bind the growth factor with an affinity that was 5-fold greater than the free aptamer. We also observed that the aptamer displayed by the supramolecular nanostructures was eight times more nuclease-resistant than the free aptamer. In order to highlight the therapeutic potential of these supramolecular systems, we demonstrated the improved inhibition of proliferation when the growth factor was bound to aptamers displayed by the nanofibers.

Original languageEnglish
Pages (from-to)2955-2963
Number of pages9
JournalACS Applied Bio Materials
Volume2
Issue number7
DOIs
Publication statusPublished - Jun 18 2019

Fingerprint

Supramolecular chemistry
Nanofibers
Amphiphiles
Nanotechnology
Oligonucleotides
Biocompatible Materials
Biomaterials
Self assembly
Peptides
Aspect ratio
DNA
Intercellular Signaling Peptides and Proteins
Nanostructures
Single-Stranded DNA
Platelet-Derived Growth Factor
Platelets
Display devices
Proteins
Degradation
Molecules

Keywords

  • aptamer
  • biomaterials
  • hybrid
  • nanotechnology
  • peptide amphiphile
  • self-assembly

ASJC Scopus subject areas

  • Biomaterials
  • Chemistry(all)
  • Biomedical Engineering
  • Biochemistry, medical

Cite this

DNA-Peptide Amphiphile Nanofibers Enhance Aptamer Function. / Serrano, Christopher M.; Freeman, Ronit; Godbe, Jacqueline; Lewis, Jacob A.; Stupp, Samuel I.

In: ACS Applied Bio Materials, Vol. 2, No. 7, 18.06.2019, p. 2955-2963.

Research output: Contribution to journalArticle

Serrano, Christopher M. ; Freeman, Ronit ; Godbe, Jacqueline ; Lewis, Jacob A. ; Stupp, Samuel I. / DNA-Peptide Amphiphile Nanofibers Enhance Aptamer Function. In: ACS Applied Bio Materials. 2019 ; Vol. 2, No. 7. pp. 2955-2963.
@article{14a47dac453d487c873c7223f1026e38,
title = "DNA-Peptide Amphiphile Nanofibers Enhance Aptamer Function",
abstract = "The single-stranded DNA oligonucleotides known as aptamers have the capacity to bind proteins and other molecules and offer great therapeutic potential. Further work is required to optimize their function and to diminish their susceptibility to nuclease degradation. We report here on the synthesis and supramolecular self-assembly of DNA-peptide amphiphiles that form high aspect ratio nanofibers and display aptamers for the platelet-derived growth factor. The nanofibers were found to bind the growth factor with an affinity that was 5-fold greater than the free aptamer. We also observed that the aptamer displayed by the supramolecular nanostructures was eight times more nuclease-resistant than the free aptamer. In order to highlight the therapeutic potential of these supramolecular systems, we demonstrated the improved inhibition of proliferation when the growth factor was bound to aptamers displayed by the nanofibers.",
keywords = "aptamer, biomaterials, hybrid, nanotechnology, peptide amphiphile, self-assembly",
author = "Serrano, {Christopher M.} and Ronit Freeman and Jacqueline Godbe and Lewis, {Jacob A.} and Stupp, {Samuel I}",
year = "2019",
month = "6",
day = "18",
doi = "10.1021/acsabm.9b00310",
language = "English",
volume = "2",
pages = "2955--2963",
journal = "ACS Applied Bio Materials",
issn = "2576-6422",
publisher = "American Chemical Society",
number = "7",

}

TY - JOUR

T1 - DNA-Peptide Amphiphile Nanofibers Enhance Aptamer Function

AU - Serrano, Christopher M.

AU - Freeman, Ronit

AU - Godbe, Jacqueline

AU - Lewis, Jacob A.

AU - Stupp, Samuel I

PY - 2019/6/18

Y1 - 2019/6/18

N2 - The single-stranded DNA oligonucleotides known as aptamers have the capacity to bind proteins and other molecules and offer great therapeutic potential. Further work is required to optimize their function and to diminish their susceptibility to nuclease degradation. We report here on the synthesis and supramolecular self-assembly of DNA-peptide amphiphiles that form high aspect ratio nanofibers and display aptamers for the platelet-derived growth factor. The nanofibers were found to bind the growth factor with an affinity that was 5-fold greater than the free aptamer. We also observed that the aptamer displayed by the supramolecular nanostructures was eight times more nuclease-resistant than the free aptamer. In order to highlight the therapeutic potential of these supramolecular systems, we demonstrated the improved inhibition of proliferation when the growth factor was bound to aptamers displayed by the nanofibers.

AB - The single-stranded DNA oligonucleotides known as aptamers have the capacity to bind proteins and other molecules and offer great therapeutic potential. Further work is required to optimize their function and to diminish their susceptibility to nuclease degradation. We report here on the synthesis and supramolecular self-assembly of DNA-peptide amphiphiles that form high aspect ratio nanofibers and display aptamers for the platelet-derived growth factor. The nanofibers were found to bind the growth factor with an affinity that was 5-fold greater than the free aptamer. We also observed that the aptamer displayed by the supramolecular nanostructures was eight times more nuclease-resistant than the free aptamer. In order to highlight the therapeutic potential of these supramolecular systems, we demonstrated the improved inhibition of proliferation when the growth factor was bound to aptamers displayed by the nanofibers.

KW - aptamer

KW - biomaterials

KW - hybrid

KW - nanotechnology

KW - peptide amphiphile

KW - self-assembly

UR - http://www.scopus.com/inward/record.url?scp=85070556261&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070556261&partnerID=8YFLogxK

U2 - 10.1021/acsabm.9b00310

DO - 10.1021/acsabm.9b00310

M3 - Article

VL - 2

SP - 2955

EP - 2963

JO - ACS Applied Bio Materials

JF - ACS Applied Bio Materials

SN - 2576-6422

IS - 7

ER -