DNAzyme-Based Logic Gate-Mediated DNA Self-Assembly

Cheng Zhang, Jing Yang, Shuoxing Jiang, Yan Liu, Hao Yan

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Controlling DNA self-assembly processes using rationally designed logic gates is a major goal of DNA-based nanotechnology and programming. Such controls could facilitate the hierarchical engineering of complex nanopatterns responding to various molecular triggers or inputs. Here, we demonstrate the use of a series of DNAzyme-based logic gates to control DNA tile self-assembly onto a prescribed DNA origami frame. Logic systems such as "YES," "OR," "AND," and "logic switch" are implemented based on DNAzyme-mediated tile recognition with the DNA origami frame. DNAzyme is designed to play two roles: (1) as an intermediate messenger to motivate downstream reactions and (2) as a final trigger to report fluorescent signals, enabling information relay between the DNA origami-framed tile assembly and fluorescent signaling. The results of this study demonstrate the plausibility of DNAzyme-mediated hierarchical self-assembly and provide new tools for generating dynamic and responsive self-assembly systems.

Original languageEnglish
Pages (from-to)736-741
Number of pages6
JournalNano Letters
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 13 2016

Fingerprint

Catalytic DNA
Logic gates
Self assembly
logic
self assembly
DNA
deoxyribonucleic acid
tiles
Tile
actuators
relay
nanotechnology
programming
Nanotechnology
switches
assembly
Switches
engineering

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

Cite this

DNAzyme-Based Logic Gate-Mediated DNA Self-Assembly. / Zhang, Cheng; Yang, Jing; Jiang, Shuoxing; Liu, Yan; Yan, Hao.

In: Nano Letters, Vol. 16, No. 1, 13.01.2016, p. 736-741.

Research output: Contribution to journalArticle

Zhang, Cheng ; Yang, Jing ; Jiang, Shuoxing ; Liu, Yan ; Yan, Hao. / DNAzyme-Based Logic Gate-Mediated DNA Self-Assembly. In: Nano Letters. 2016 ; Vol. 16, No. 1. pp. 736-741.
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