Droplet streams for serial crystallography of proteins

U. Weierstall, R. B. Doak, J. C H Spence, D. Starodub, D. Shapiro, P. Kennedy, J. Warner, G. G. Hembree, Petra Fromme, H. N. Chapman

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Serial diffraction of proteins requires an injection method to deliver analyte molecules-preferably uncharged, fully hydrated, spatially oriented, and with high flux-into a focused probe beam of electrons or X-rays that is only a few tens of microns in diameter. This work examines conventional Rayleigh sources and electrospray-assisted Rayleigh sources as to their suitability for this task. A comparison is made and conclusions drawn on the basis of time-resolved optical images of the droplet streams produced by these sources. Straight-line periodic streams of monodisperse droplets were generated with both sources, achieving droplet diameters of 4 and 1 micrometer, respectively, for the conventional and electrospray-assisted versions. Shrinkage of droplets by evaporation is discussed and quantified. It is shown experimentally that proteins pass undamaged through a conventional Rayleigh droplet source.

Original languageEnglish
Pages (from-to)675-689
Number of pages15
JournalExperiments in Fluids
Volume44
Issue number5
DOIs
Publication statusPublished - May 2008

Fingerprint

Crystallography
crystallography
proteins
Proteins
shrinkage
micrometers
Evaporation
Diffraction
evaporation
injection
Fluxes
X rays
Molecules
Electrons
probes
diffraction
molecules
electrons
x rays

ASJC Scopus subject areas

  • Mechanical Engineering
  • Fluid Flow and Transfer Processes
  • Mechanics of Materials
  • Computational Mechanics

Cite this

Weierstall, U., Doak, R. B., Spence, J. C. H., Starodub, D., Shapiro, D., Kennedy, P., ... Chapman, H. N. (2008). Droplet streams for serial crystallography of proteins. Experiments in Fluids, 44(5), 675-689. https://doi.org/10.1007/s00348-007-0426-8

Droplet streams for serial crystallography of proteins. / Weierstall, U.; Doak, R. B.; Spence, J. C H; Starodub, D.; Shapiro, D.; Kennedy, P.; Warner, J.; Hembree, G. G.; Fromme, Petra; Chapman, H. N.

In: Experiments in Fluids, Vol. 44, No. 5, 05.2008, p. 675-689.

Research output: Contribution to journalArticle

Weierstall, U, Doak, RB, Spence, JCH, Starodub, D, Shapiro, D, Kennedy, P, Warner, J, Hembree, GG, Fromme, P & Chapman, HN 2008, 'Droplet streams for serial crystallography of proteins', Experiments in Fluids, vol. 44, no. 5, pp. 675-689. https://doi.org/10.1007/s00348-007-0426-8
Weierstall U, Doak RB, Spence JCH, Starodub D, Shapiro D, Kennedy P et al. Droplet streams for serial crystallography of proteins. Experiments in Fluids. 2008 May;44(5):675-689. https://doi.org/10.1007/s00348-007-0426-8
Weierstall, U. ; Doak, R. B. ; Spence, J. C H ; Starodub, D. ; Shapiro, D. ; Kennedy, P. ; Warner, J. ; Hembree, G. G. ; Fromme, Petra ; Chapman, H. N. / Droplet streams for serial crystallography of proteins. In: Experiments in Fluids. 2008 ; Vol. 44, No. 5. pp. 675-689.
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