Dynamic in vivo biocompatibility of angiogenic peptide amphiphile nanofibers

Shahram Ghanaati, Matthew J. Webber, Ronald E. Unger, Carina Orth, James F. Hulvat, Sarah E. Kiehna, Mike Barbeck, Angela Rasic, Samuel I Stupp, C. James Kirkpatrick

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Biomaterials that promote angiogenesis have great potential in regenerative medicine for rapid revascularization of damaged tissue, survival of transplanted cells, and healing of chronic wounds. Supramolecular nanofibers formed by self-assembly of a heparin-binding peptide amphiphile and heparan sulfate-like glycosaminoglycans were evaluated here using a dorsal skinfold chamber model to dynamically monitor the interaction between the nanofiber gel and the microcirculation, representing a novel application of this model. We paired this model with a conventional subcutaneous implantation model for static histological assessment of the interactions between the gel and host tissue. In the static analysis, the heparan sulfate-containing nanofiber gels were found to persist in the tissue for up to 30 days and revealed excellent biocompatibility. Strikingly, as the nanofiber gel biodegraded, we observed the formation of a de novo vascularized connective tissue. In the dynamic experiments using the dorsal skinfold chamber, the material again demonstrated good biocompatibility, with minimal dilation of the microcirculation and only a few adherent leukocytes, monitored through intravital fluorescence microscopy. The new application of the dorsal skinfold model corroborated our findings from the traditional static histology, demonstrating the potential use of this technique to dynamically evaluate the biocompatibility of materials. The observed biocompatibility and development of new vascularized tissue using both techniques demonstrates the potential of these angiogenesis-promoting materials for a host of regenerative strategies.

Original languageEnglish
Pages (from-to)6202-6212
Number of pages11
JournalBiomaterials
Volume30
Issue number31
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Angiogenic Proteins
Nanofibers
Amphiphiles
Biocompatibility
Peptides
Gels
Tissue
Heparitin Sulfate
Microcirculation
Tissue Survival
Regenerative Medicine
Biocompatible Materials
Glycosaminoglycans
Fluorescence Microscopy
Histology
Connective Tissue
Wound Healing
Fluorescence microscopy
Heparin
Static analysis

Keywords

  • Angiogenesis
  • Biocompatibility
  • Peptide amphiphile
  • Regenerative medicine
  • Self-assembly

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Ghanaati, S., Webber, M. J., Unger, R. E., Orth, C., Hulvat, J. F., Kiehna, S. E., ... Kirkpatrick, C. J. (2009). Dynamic in vivo biocompatibility of angiogenic peptide amphiphile nanofibers. Biomaterials, 30(31), 6202-6212. https://doi.org/10.1016/j.biomaterials.2009.07.063

Dynamic in vivo biocompatibility of angiogenic peptide amphiphile nanofibers. / Ghanaati, Shahram; Webber, Matthew J.; Unger, Ronald E.; Orth, Carina; Hulvat, James F.; Kiehna, Sarah E.; Barbeck, Mike; Rasic, Angela; Stupp, Samuel I; Kirkpatrick, C. James.

In: Biomaterials, Vol. 30, No. 31, 10.2009, p. 6202-6212.

Research output: Contribution to journalArticle

Ghanaati, S, Webber, MJ, Unger, RE, Orth, C, Hulvat, JF, Kiehna, SE, Barbeck, M, Rasic, A, Stupp, SI & Kirkpatrick, CJ 2009, 'Dynamic in vivo biocompatibility of angiogenic peptide amphiphile nanofibers', Biomaterials, vol. 30, no. 31, pp. 6202-6212. https://doi.org/10.1016/j.biomaterials.2009.07.063
Ghanaati S, Webber MJ, Unger RE, Orth C, Hulvat JF, Kiehna SE et al. Dynamic in vivo biocompatibility of angiogenic peptide amphiphile nanofibers. Biomaterials. 2009 Oct;30(31):6202-6212. https://doi.org/10.1016/j.biomaterials.2009.07.063
Ghanaati, Shahram ; Webber, Matthew J. ; Unger, Ronald E. ; Orth, Carina ; Hulvat, James F. ; Kiehna, Sarah E. ; Barbeck, Mike ; Rasic, Angela ; Stupp, Samuel I ; Kirkpatrick, C. James. / Dynamic in vivo biocompatibility of angiogenic peptide amphiphile nanofibers. In: Biomaterials. 2009 ; Vol. 30, No. 31. pp. 6202-6212.
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