The proton-translocating ATPase from chloroplasts, CF0F1, was isolated, purified and reconstitutedinto asolectin liposomes. The effect of the energy transfer inhibitor, tentoxin, on different functions of the enzyme was investigated. Tentoxin does not inhibit the nucleotide release during energization by a pH Δψ jump, i.e. the activation of the enzyme is not influenced. ATP synthesis driven by a pH Δψ T jump and multi-site ATP hydrolysis are completely inhibited by tentoxin, whereas uni-site ATP hydrolysis is not influenced.
|Number of pages||6|
|Journal||Zeitschrift fur Naturforschung - Section C Journal of Biosciences|
|Publication status||Published - Apr 1 1992|
- Enzyme Kinetics
- Uni-Site Catalysis
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)