Effects of bilayer surface charge density on molecular adsorption and transport across liposome bilayers

Yan Liu, Elsa C.Y. Yan, Kenneth B. Eisenthal

Research output: Contribution to journalArticle

59 Citations (Scopus)


Second harmonic generation (SHG) was used to study both the adsorption of malachite green (MG), a positively charged organic dye, onto liposomes of different lipid compositions, and the transport kinetics of MG across the liposome bilayer in real time. We found that the dye adsorption increased linearly with the fraction of negatively charged lipids in the bilayer. Similarly, the transport rate constant for crossing the bilayer increased linearly with the fraction of charged lipid in the bilayer.

Original languageEnglish
Pages (from-to)1004-1012
Number of pages9
JournalBiophysical journal
Issue number2
Publication statusPublished - Jan 1 2001


ASJC Scopus subject areas

  • Biophysics

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