Efficient light harvesting in a dark, hot, acidic environment

The structure and function of PSI-LHCI from Galdieria sulphuraria

Balakumar Thangaraj, Craig C. Jolley, Iosifina Sarrou, Jelle B. Bultema, Jason Greyslak, Julian P. Whitelegge, Su Lin, Roman Kouřil, Rajagopal Subramanyam, Egbert J. Boekema, Petra Fromme

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Photosystem I-light harvesting complex I (PSI-LHCI) was isolated from the thermoacidophilic red alga Galdieria sulphuraria, and its structure, composition, and light-harvesting function were characterized by electron microscopy, mass spectrometry, and ultrafast optical spectroscopy. The results show that Galdieria PSI is a monomer with core features similar to those of PSI from green algae, but with significant differences in shape and size. A comparison with the crystal structure of higher plant (pea) PSI-LHCI indicates that Galdieria PSI binds seven to nine light-harvesting proteins. Results from ultrafast optical spectroscopy show that the functional coupling of the LHCI proteins to the PSI core is tighter than in other eukaryotic PSI-LHCI systems reported thus far. This tight coupling helps Galdieria perform efficient light harvesting under the low-light conditions present in its natural endolithic habitat.

Original languageEnglish
Pages (from-to)135-143
Number of pages9
JournalBiophysical Journal
Volume100
Issue number1
DOIs
Publication statusPublished - Jan 5 2011

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Photosystem I Protein Complex
Light
Spectrum Analysis
Rhodophyta
Plant Structures
Chlorophyta
Peas
Ecosystem
Mass Spectrometry
Electron Microscopy
Proteins

ASJC Scopus subject areas

  • Biophysics

Cite this

Efficient light harvesting in a dark, hot, acidic environment : The structure and function of PSI-LHCI from Galdieria sulphuraria. / Thangaraj, Balakumar; Jolley, Craig C.; Sarrou, Iosifina; Bultema, Jelle B.; Greyslak, Jason; Whitelegge, Julian P.; Lin, Su; Kouřil, Roman; Subramanyam, Rajagopal; Boekema, Egbert J.; Fromme, Petra.

In: Biophysical Journal, Vol. 100, No. 1, 05.01.2011, p. 135-143.

Research output: Contribution to journalArticle

Thangaraj, B, Jolley, CC, Sarrou, I, Bultema, JB, Greyslak, J, Whitelegge, JP, Lin, S, Kouřil, R, Subramanyam, R, Boekema, EJ & Fromme, P 2011, 'Efficient light harvesting in a dark, hot, acidic environment: The structure and function of PSI-LHCI from Galdieria sulphuraria', Biophysical Journal, vol. 100, no. 1, pp. 135-143. https://doi.org/10.1016/j.bpj.2010.09.069
Thangaraj, Balakumar ; Jolley, Craig C. ; Sarrou, Iosifina ; Bultema, Jelle B. ; Greyslak, Jason ; Whitelegge, Julian P. ; Lin, Su ; Kouřil, Roman ; Subramanyam, Rajagopal ; Boekema, Egbert J. ; Fromme, Petra. / Efficient light harvesting in a dark, hot, acidic environment : The structure and function of PSI-LHCI from Galdieria sulphuraria. In: Biophysical Journal. 2011 ; Vol. 100, No. 1. pp. 135-143.
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