Engineered photosystem II reaction centers optimize photochemistry versus photoprotection at different solar intensities

David J. Vinyard, Javier Gimpel, Gennady M. Ananyev, Stephen P. Mayfield, G Charles Dismukes

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The D1 protein of Photosystem II (PSII) provides most of the ligating amino acid residues for the Mn4CaO5 water-oxidizing complex (WOC) and half of the reaction center cofactors, and it is present as two isoforms in the cyanobacterium Synechococcus elongatus PCC 7942. These isoforms, D1:1 and D1:2, confer functional advantages for photosynthetic growth at low and high light intensities, respectively. D1:1, D1:2, and seven point mutations in the D1:2 background that are native to D1:1 were expressed in the green alga Chlamydomonas reinhardtii. We used these nine strains to show that those strains that confer a higher yield of PSII charge separation under light-limiting conditions (where charge recombination is significant) have less efficient photochemical turnover, measured in terms of both a lower WOC turnover probability and a longer WOC cycle period. Conversely, these same strains under light saturation (where charge recombination does not compete) confer a correspondingly faster O2 evolution rate and greater protection against photoinhibition. Taken together, the data clearly establish that PSII primary charge separation is a trade-off between photochemical productivity (water oxidation and plastoquinone reduction) and charge recombination (photoprotection). These trade-offs add up to a significant growth advantage for the two natural isoforms. These insights provide fundamental design principles for engineering of PSII reaction centers with optimal photochemical efficiencies for growth at low versus high light intensities.

Original languageEnglish
Pages (from-to)4048-4055
Number of pages8
JournalJournal of the American Chemical Society
Volume136
Issue number10
DOIs
Publication statusPublished - Mar 12 2014

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Photochemistry
Photosystem II Protein Complex
Photochemical reactions
High intensity light
Genetic Recombination
Light
Protein Isoforms
Water
Growth
Plastoquinone
Synechococcus
Chlamydomonas reinhardtii
Chlorophyta
Cyanobacteria
Algae
Point Mutation
Amino acids
Productivity
Proteins
Amino Acids

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry
  • Medicine(all)

Cite this

Engineered photosystem II reaction centers optimize photochemistry versus photoprotection at different solar intensities. / Vinyard, David J.; Gimpel, Javier; Ananyev, Gennady M.; Mayfield, Stephen P.; Dismukes, G Charles.

In: Journal of the American Chemical Society, Vol. 136, No. 10, 12.03.2014, p. 4048-4055.

Research output: Contribution to journalArticle

Vinyard, David J. ; Gimpel, Javier ; Ananyev, Gennady M. ; Mayfield, Stephen P. ; Dismukes, G Charles. / Engineered photosystem II reaction centers optimize photochemistry versus photoprotection at different solar intensities. In: Journal of the American Chemical Society. 2014 ; Vol. 136, No. 10. pp. 4048-4055.
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