Enhanced oligonucleotide binding to self-assembled nanofibers

Mustafa O. Guler, Jonathan K. Pokorski, Daniel H. Appella, Samuel I Stupp

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A peptide nucleic acid/peptide amphiphile conjugate (PNA-PA) that self-assembles into fiber-shaped nanostructures was designed to bind oligonucleotides with high affinity and specificity. Oligonucleotide binding to PNA-PA nanofibers was studied using fluorescence polarization, and thermal stability was examined by UV-vis measurement of duplex melting temperatures. The self-assembled PNA-PA DNA system was observed to bind more strongly than the corresponding DNA-DNA duplex. We also observed single base specificity with a 16°C in thermal stability. As expected from the previous PNA studies, PNA-PA RNA binding is also stronger than the corresponding RNA-RNA duplex.

Original languageEnglish
Pages (from-to)501-503
Number of pages3
JournalBioconjugate Chemistry
Volume16
Issue number3
DOIs
Publication statusPublished - May 2005

Fingerprint

Peptide Nucleic Acids
Nanofibers
Amphiphiles
Oligonucleotides
Peptides
RNA
DNA
Thermodynamic stability
Hot Temperature
Fluorescence Polarization
Nanostructures
Freezing
Melting point
Fluorescence
Polarization
Temperature
Fibers

ASJC Scopus subject areas

  • Chemistry(all)
  • Organic Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Enhanced oligonucleotide binding to self-assembled nanofibers. / Guler, Mustafa O.; Pokorski, Jonathan K.; Appella, Daniel H.; Stupp, Samuel I.

In: Bioconjugate Chemistry, Vol. 16, No. 3, 05.2005, p. 501-503.

Research output: Contribution to journalArticle

Guler, Mustafa O. ; Pokorski, Jonathan K. ; Appella, Daniel H. ; Stupp, Samuel I. / Enhanced oligonucleotide binding to self-assembled nanofibers. In: Bioconjugate Chemistry. 2005 ; Vol. 16, No. 3. pp. 501-503.
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