Abstract
Hydrogen is produced by a [NiFe]-hydrogenase in the cyanobacterium Arthrospira (Spirulina) maxima during autofermentation of photosynthetically accumulated glycogen under dark anaerobic conditions. Herein we show that elimination of H2 backpressure by continuous H2 removal (" milking" ) can significantly increase the yield of H2 in this strain. We show that " milking" by continuous selective consumption of H2 using an electrochemical cell produces the maximum increase in H2 yield (11-fold) and H2 rate (3.4-fold), which is considerably larger than through " milking" by non-selective dilution of the biomass in media (increases H2 yield 3.7-fold and rate 3.1-fold). Exhaustive autofermentation under electrochemical milking conditions consumes >98% of glycogen and 27.6% of biomass over 7-8days and extracts 39% of the energy content in glycogen as H2. Non-selective dilution stimulates H2 production by shifting intracellular equilibria competing for NADH from excreted products and terminal electron sinks into H2 production. Adding a mixture of the carbon fermentative products shifts the equilibria towards reactants, resulting in increased intracellular NADH and an increased H2 yield (1.4-fold). H2 production is sustained for a period of time up to 7days, after which the PSII activity of the cells decreases by 80-90%, but can be restored by regeneration under photoautotrophic growth.
| Original language | English |
|---|---|
| Pages (from-to) | 97-104 |
| Number of pages | 8 |
| Journal | Journal of Biotechnology |
| Volume | 162 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Nov 30 2012 |
Fingerprint
Keywords
- [NiFe] hydrogenase
- Arthrospira (Spirulina) maxima
- Biofuel
- Cyanobacteria
- Excreted products
- Fermentation
- Hydrogen
- Milking
- NADH
- NADPH
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Enhancing biological hydrogen production from cyanobacteria by removal of excreted products. / Ananyev, Gennady M.; Skizim, Nicholas J.; Dismukes, G Charles.
In: Journal of Biotechnology, Vol. 162, No. 1, 30.11.2012, p. 97-104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhancing biological hydrogen production from cyanobacteria by removal of excreted products
AU - Ananyev, Gennady M.
AU - Skizim, Nicholas J.
AU - Dismukes, G Charles
PY - 2012/11/30
Y1 - 2012/11/30
N2 - Hydrogen is produced by a [NiFe]-hydrogenase in the cyanobacterium Arthrospira (Spirulina) maxima during autofermentation of photosynthetically accumulated glycogen under dark anaerobic conditions. Herein we show that elimination of H2 backpressure by continuous H2 removal (" milking" ) can significantly increase the yield of H2 in this strain. We show that " milking" by continuous selective consumption of H2 using an electrochemical cell produces the maximum increase in H2 yield (11-fold) and H2 rate (3.4-fold), which is considerably larger than through " milking" by non-selective dilution of the biomass in media (increases H2 yield 3.7-fold and rate 3.1-fold). Exhaustive autofermentation under electrochemical milking conditions consumes >98% of glycogen and 27.6% of biomass over 7-8days and extracts 39% of the energy content in glycogen as H2. Non-selective dilution stimulates H2 production by shifting intracellular equilibria competing for NADH from excreted products and terminal electron sinks into H2 production. Adding a mixture of the carbon fermentative products shifts the equilibria towards reactants, resulting in increased intracellular NADH and an increased H2 yield (1.4-fold). H2 production is sustained for a period of time up to 7days, after which the PSII activity of the cells decreases by 80-90%, but can be restored by regeneration under photoautotrophic growth.
AB - Hydrogen is produced by a [NiFe]-hydrogenase in the cyanobacterium Arthrospira (Spirulina) maxima during autofermentation of photosynthetically accumulated glycogen under dark anaerobic conditions. Herein we show that elimination of H2 backpressure by continuous H2 removal (" milking" ) can significantly increase the yield of H2 in this strain. We show that " milking" by continuous selective consumption of H2 using an electrochemical cell produces the maximum increase in H2 yield (11-fold) and H2 rate (3.4-fold), which is considerably larger than through " milking" by non-selective dilution of the biomass in media (increases H2 yield 3.7-fold and rate 3.1-fold). Exhaustive autofermentation under electrochemical milking conditions consumes >98% of glycogen and 27.6% of biomass over 7-8days and extracts 39% of the energy content in glycogen as H2. Non-selective dilution stimulates H2 production by shifting intracellular equilibria competing for NADH from excreted products and terminal electron sinks into H2 production. Adding a mixture of the carbon fermentative products shifts the equilibria towards reactants, resulting in increased intracellular NADH and an increased H2 yield (1.4-fold). H2 production is sustained for a period of time up to 7days, after which the PSII activity of the cells decreases by 80-90%, but can be restored by regeneration under photoautotrophic growth.
KW - [NiFe] hydrogenase
KW - Arthrospira (Spirulina) maxima
KW - Biofuel
KW - Cyanobacteria
KW - Excreted products
KW - Fermentation
KW - Hydrogen
KW - Milking
KW - NADH
KW - NADPH
UR - http://www.scopus.com/inward/record.url?scp=84867629221&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84867629221&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2012.03.026
DO - 10.1016/j.jbiotec.2012.03.026
M3 - Article
C2 - 22503939
AN - SCOPUS:84867629221
VL - 162
SP - 97
EP - 104
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
IS - 1
ER -