Enhancing biological hydrogen production from cyanobacteria by removal of excreted products

Gennady M. Ananyev, Nicholas J. Skizim, G. Charles Dismukes

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Hydrogen is produced by a [NiFe]-hydrogenase in the cyanobacterium Arthrospira (Spirulina) maxima during autofermentation of photosynthetically accumulated glycogen under dark anaerobic conditions. Herein we show that elimination of H2 backpressure by continuous H2 removal (" milking" ) can significantly increase the yield of H2 in this strain. We show that " milking" by continuous selective consumption of H2 using an electrochemical cell produces the maximum increase in H2 yield (11-fold) and H2 rate (3.4-fold), which is considerably larger than through " milking" by non-selective dilution of the biomass in media (increases H2 yield 3.7-fold and rate 3.1-fold). Exhaustive autofermentation under electrochemical milking conditions consumes >98% of glycogen and 27.6% of biomass over 7-8days and extracts 39% of the energy content in glycogen as H2. Non-selective dilution stimulates H2 production by shifting intracellular equilibria competing for NADH from excreted products and terminal electron sinks into H2 production. Adding a mixture of the carbon fermentative products shifts the equilibria towards reactants, resulting in increased intracellular NADH and an increased H2 yield (1.4-fold). H2 production is sustained for a period of time up to 7days, after which the PSII activity of the cells decreases by 80-90%, but can be restored by regeneration under photoautotrophic growth.

Original languageEnglish
Pages (from-to)97-104
Number of pages8
JournalJournal of Biotechnology
Volume162
Issue number1
DOIs
Publication statusPublished - Nov 30 2012

Keywords

  • Arthrospira (Spirulina) maxima
  • Biofuel
  • Cyanobacteria
  • Excreted products
  • Fermentation
  • Hydrogen
  • Milking
  • NADH
  • NADPH
  • [NiFe] hydrogenase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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