Abstract
Synthetic methods and biocatalytic activities of new classes of heterogeneous biocatalysts by immobilizing flavin-containing monooxygenase on corrugated and nanoporous silica nanospheres are reported. The nanoporous and corrugated silica nanospheres are synthesized by etching silica nanospheres with aqueous KOH solution. The etched nanospheres are proven to have increased surface area, corrugated, cage-like external surfaces, and, most importantly, more accessible and well-suited surfaces to immobilize bigger molecules, such as enzymes. Furthermore, the etched silica nanospheres contain hydrophilic and silanol groups that are conducive for anchoring enzymes. By utilizing the structures of the etched silica nanospheres, effective immobilization of flavin-containing monooxygenase 1 (FMO1) is demonstrated. The FMO1 immobilized etched silica nanospheres have shown efficient and recyclable biocatalytic activity for nicotine oxidation. Synthetic methods and biocatalytic activities of new classes of heterogeneous biocatalysts by immobilizing flavin-containing monooxygenase on corrugated and nanoporous silica nanospheres are reported. By using the structures of the etched silica nanospheres, effective immobilization of flavin-containing monooxygenase 1 (FMO1) is demonstrated. The FMO1 immobilized etched silica nanospheres have shown efficient and recyclable biocatalytic activity for nicotine oxidation.
| Original language | English |
|---|---|
| Pages (from-to) | 1004-1010 |
| Number of pages | 7 |
| Journal | ChemCatChem |
| Volume | 2 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Aug 9 2010 |
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Keywords
- Biocatalysis
- Enzymes
- Heterogeneous catalysis
- Immobilization
- Nanostructures
ASJC Scopus subject areas
- Inorganic Chemistry
- Organic Chemistry
- Physical and Theoretical Chemistry
- Catalysis
Cite this
Entrapping Flavin-Containing Monooxygenase on Corrugated Silica Nanospheres and their Recyclable Biocatalytic Activities. / Biradar, Archana A.; Biradar, Ankush V.; Asefa, Teddy.
In: ChemCatChem, Vol. 2, No. 8, 09.08.2010, p. 1004-1010.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Entrapping Flavin-Containing Monooxygenase on Corrugated Silica Nanospheres and their Recyclable Biocatalytic Activities
AU - Biradar, Archana A.
AU - Biradar, Ankush V.
AU - Asefa, Teddy
PY - 2010/8/9
Y1 - 2010/8/9
N2 - Synthetic methods and biocatalytic activities of new classes of heterogeneous biocatalysts by immobilizing flavin-containing monooxygenase on corrugated and nanoporous silica nanospheres are reported. The nanoporous and corrugated silica nanospheres are synthesized by etching silica nanospheres with aqueous KOH solution. The etched nanospheres are proven to have increased surface area, corrugated, cage-like external surfaces, and, most importantly, more accessible and well-suited surfaces to immobilize bigger molecules, such as enzymes. Furthermore, the etched silica nanospheres contain hydrophilic and silanol groups that are conducive for anchoring enzymes. By utilizing the structures of the etched silica nanospheres, effective immobilization of flavin-containing monooxygenase 1 (FMO1) is demonstrated. The FMO1 immobilized etched silica nanospheres have shown efficient and recyclable biocatalytic activity for nicotine oxidation. Synthetic methods and biocatalytic activities of new classes of heterogeneous biocatalysts by immobilizing flavin-containing monooxygenase on corrugated and nanoporous silica nanospheres are reported. By using the structures of the etched silica nanospheres, effective immobilization of flavin-containing monooxygenase 1 (FMO1) is demonstrated. The FMO1 immobilized etched silica nanospheres have shown efficient and recyclable biocatalytic activity for nicotine oxidation.
AB - Synthetic methods and biocatalytic activities of new classes of heterogeneous biocatalysts by immobilizing flavin-containing monooxygenase on corrugated and nanoporous silica nanospheres are reported. The nanoporous and corrugated silica nanospheres are synthesized by etching silica nanospheres with aqueous KOH solution. The etched nanospheres are proven to have increased surface area, corrugated, cage-like external surfaces, and, most importantly, more accessible and well-suited surfaces to immobilize bigger molecules, such as enzymes. Furthermore, the etched silica nanospheres contain hydrophilic and silanol groups that are conducive for anchoring enzymes. By utilizing the structures of the etched silica nanospheres, effective immobilization of flavin-containing monooxygenase 1 (FMO1) is demonstrated. The FMO1 immobilized etched silica nanospheres have shown efficient and recyclable biocatalytic activity for nicotine oxidation. Synthetic methods and biocatalytic activities of new classes of heterogeneous biocatalysts by immobilizing flavin-containing monooxygenase on corrugated and nanoporous silica nanospheres are reported. By using the structures of the etched silica nanospheres, effective immobilization of flavin-containing monooxygenase 1 (FMO1) is demonstrated. The FMO1 immobilized etched silica nanospheres have shown efficient and recyclable biocatalytic activity for nicotine oxidation.
KW - Biocatalysis
KW - Enzymes
KW - Heterogeneous catalysis
KW - Immobilization
KW - Nanostructures
UR - http://www.scopus.com/inward/record.url?scp=79957693530&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79957693530&partnerID=8YFLogxK
U2 - 10.1002/cctc.200900253
DO - 10.1002/cctc.200900253
M3 - Article
AN - SCOPUS:79957693530
VL - 2
SP - 1004
EP - 1010
JO - ChemCatChem
JF - ChemCatChem
SN - 1867-3880
IS - 8
ER -