Enzymatic Biodegradability of Pristine and Functionalized Transition Metal Dichalcogenide MoS2 Nanosheets

Rajendra Kurapati, Laura Muzi, Aritz Perez Ruiz de Garibay, Julie Russier, Damien Voiry, Isabella A. Vacchi, Manish Chhowalla, Alberto Bianco

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

2D transition metal dichalcogenide MoS2 nanosheets are increasingly attracting interests due to their promising applications in materials science and biomedicine. However, their biocompatibility and their biodegradability have not been thoroughly studied yet. Here, the biodegradability of exfoliated pristine and covalently functionalized MoS2 (f-MoS2) is investigated. First, biodegradability of these nanomaterials is evaluated using plant horseradish peroxidase and human myeloperoxidase. The results reveal that the enzymatic degradability rate of MoS2 and f-MoS2 is slower than in the case of the simple treatment with H2O2 alone. In parallel, high biocompatibility of both pristine and f-MoS2 nanosheets is found up to 100 µg mL−1 in both cell lines (HeLa and Raw264.7) and primary immune cells. In addition, no immune cell activation and minimal pro-inflammatory cytokine release are observed in RAW264.7 and human monocyte-derived macrophages, suggesting a negligible cellular impact of such materials. Furthermore, the effects of degraded MoS2 and partially degraded f-MoS2 products on cell viability and activation are studied in cancer and immune cells. A certain cytotoxicity is measured at the highest concentrations. Finally, to prove that the cellular impact is due to cell uptake, the internalization of both pristine and functionalized MoS2 in cancer and primary immune cells is assessed.

Original languageEnglish
Article number1605176
JournalAdvanced Functional Materials
Volume27
Issue number7
DOIs
Publication statusPublished - Feb 17 2017

Fingerprint

biodegradability
Nanosheets
Biodegradability
Transition metals
transition metals
Biocompatibility
cells
Chemical activation
Cells
biocompatibility
Macrophages
Materials science
Horseradish Peroxidase
Cytotoxicity
Nanostructured materials
Peroxidase
cancer
activation
monocytes
macrophages

Keywords

  • cytotoxicity
  • degradation
  • graphene-related materials
  • molybdenum disulfide
  • peroxidases

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Condensed Matter Physics
  • Electrochemistry

Cite this

Kurapati, R., Muzi, L., de Garibay, A. P. R., Russier, J., Voiry, D., Vacchi, I. A., ... Bianco, A. (2017). Enzymatic Biodegradability of Pristine and Functionalized Transition Metal Dichalcogenide MoS2 Nanosheets. Advanced Functional Materials, 27(7), [1605176]. https://doi.org/10.1002/adfm.201605176

Enzymatic Biodegradability of Pristine and Functionalized Transition Metal Dichalcogenide MoS2 Nanosheets. / Kurapati, Rajendra; Muzi, Laura; de Garibay, Aritz Perez Ruiz; Russier, Julie; Voiry, Damien; Vacchi, Isabella A.; Chhowalla, Manish; Bianco, Alberto.

In: Advanced Functional Materials, Vol. 27, No. 7, 1605176, 17.02.2017.

Research output: Contribution to journalArticle

Kurapati, Rajendra ; Muzi, Laura ; de Garibay, Aritz Perez Ruiz ; Russier, Julie ; Voiry, Damien ; Vacchi, Isabella A. ; Chhowalla, Manish ; Bianco, Alberto. / Enzymatic Biodegradability of Pristine and Functionalized Transition Metal Dichalcogenide MoS2 Nanosheets. In: Advanced Functional Materials. 2017 ; Vol. 27, No. 7.
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