Enzymatic degradation of low-rank coals by a cell-free enzymatic system from Coriolus versicolor

John W. Pyne, Dorothy L. Stewart, John Linehan, Roger M. Bean, Mathew A. Powell, Richard B. Lucke, Berta L. Thomas, James A. Campbell, Bary W. Wilson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An extracellular fraction from the lignin degrading white-rot fungus, Coriolus versicolor, can biosolubilize leonardite and pretreated lignites in vitro. Leonardite is often found as an overburden to lignite deposits, and is related to humic acid, and is more oxidized than lignites. Extracts from certain fungi such as C. versicolor solubilize leonardite. The biosolubilization activity is at least in part enzymatic in nature. A protein which is at least partly responsible for the solubilization effect has been substantially purified and concentrated by ultrafiltration and ion-exchange chromatography from the broth of an agitated submerged culture of C. versicolor grown in a defined medium. An in vitro coal biosolubilization assay was used to establish the effects of various physical and chemical factors affecting enzyme activity, including temperature and the salt and buffer composition of the reaction mixture. Some initial studies on the mechanism of the enzymatic leonardite biosolubilization reaction are described.

Original languageEnglish
Pages (from-to)185-195
Number of pages11
JournalResources, Conservation and Recycling
Volume1
Issue number3-4
DOIs
Publication statusPublished - 1988

Fingerprint

coal rank
Fungi
Coal
fungus
Degradation
degradation
Enzyme activity
Lignite
solubilization
Ultrafiltration
Chromatography
ultrafiltration
Lignin
lignite
overburden
humic acid
lignin
chromatography
enzyme activity
Assays

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Management, Monitoring, Policy and Law
  • Waste Management and Disposal
  • Environmental Science(all)
  • Earth and Planetary Sciences(all)

Cite this

Enzymatic degradation of low-rank coals by a cell-free enzymatic system from Coriolus versicolor. / Pyne, John W.; Stewart, Dorothy L.; Linehan, John; Bean, Roger M.; Powell, Mathew A.; Lucke, Richard B.; Thomas, Berta L.; Campbell, James A.; Wilson, Bary W.

In: Resources, Conservation and Recycling, Vol. 1, No. 3-4, 1988, p. 185-195.

Research output: Contribution to journalArticle

Pyne, JW, Stewart, DL, Linehan, J, Bean, RM, Powell, MA, Lucke, RB, Thomas, BL, Campbell, JA & Wilson, BW 1988, 'Enzymatic degradation of low-rank coals by a cell-free enzymatic system from Coriolus versicolor', Resources, Conservation and Recycling, vol. 1, no. 3-4, pp. 185-195. https://doi.org/10.1016/0921-3449(88)90015-8
Pyne, John W. ; Stewart, Dorothy L. ; Linehan, John ; Bean, Roger M. ; Powell, Mathew A. ; Lucke, Richard B. ; Thomas, Berta L. ; Campbell, James A. ; Wilson, Bary W. / Enzymatic degradation of low-rank coals by a cell-free enzymatic system from Coriolus versicolor. In: Resources, Conservation and Recycling. 1988 ; Vol. 1, No. 3-4. pp. 185-195.
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