Evaluation of the tissue reaction to a new bilayered collagen matrix in vivo and its translation to the clinic

Shahram Ghanaati, Markus Schlee, Matthew J. Webber, Ines Willershausen, Mike Barbeck, Ela Balic, Christoph Görlach, Samuel I Stupp, Robert A. Sader, C. James Kirkpatrick

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

This study evaluates a new collagen matrix that is designed with a bilayered structure in order to promote guided tissue regeneration and integration within the host tissue. This material induced a mild tissue reaction when assessed in a murine model and was well integrated within the host tissue, persisting in the implantation bed throughout the in vivo study. A more porous layer was rapidly infiltrated by host mesenchymal cells, while a layer designed to be a barrier allowed cell attachment and host tissue integration, but at the same time remained impermeable to invading cells for the first 30 days of the study. The tissue reaction was favorable, and unlike a typical foreign body response, did not include the presence of multinucleated giant cells, lymphocytes, or granulation tissue. In the context of translation, we show preliminary results from the clinical use of this biomaterial applied to soft tissue regeneration in the treatment of gingival tissue recession and exposed roots of human teeth. Such a condition would greatly benefit from guided tissue regeneration strategies. Our findings demonstrate that this material successfully promoted the ingrowth of gingival tissue and reversed gingival tissue recession. Of particular importance is the fact that the histological evidence from these human studies corroborates our findings in the murine model, with the barrier layer preventing unspecific tissue ingrowth, as the scaffold becomes infiltrated by mesenchymal cells from adjacent tissue into the porous layer. Also in the clinical situation no multinucleated giant cells, no granulation tissue and no evidence of a marked inflammatory response were observed. In conclusion, this bilayered matrix elicits a favorable tissue reaction, demonstrates potential as a barrier for preferential tissue ingrowth, and achieves a desirable therapeutic result when applied in humans for soft tissue regeneration.

Original languageEnglish
Article number015010
JournalBiomedical Materials
Volume6
Issue number1
DOIs
Publication statusPublished - 2011

Fingerprint

Collagen
Tissue
Tissue regeneration
Guided Tissue Regeneration
Gingival Recession
Granulation
Granulation Tissue
Giant Cells
Regeneration
Tooth Root
Lymphocytes
Biocompatible Materials
Foreign Bodies
Biomaterials
Scaffolds

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Biochemistry
  • Mechanics of Materials
  • Medicine(all)

Cite this

Ghanaati, S., Schlee, M., Webber, M. J., Willershausen, I., Barbeck, M., Balic, E., ... Kirkpatrick, C. J. (2011). Evaluation of the tissue reaction to a new bilayered collagen matrix in vivo and its translation to the clinic. Biomedical Materials, 6(1), [015010]. https://doi.org/10.1088/1748-6041/6/1/015010

Evaluation of the tissue reaction to a new bilayered collagen matrix in vivo and its translation to the clinic. / Ghanaati, Shahram; Schlee, Markus; Webber, Matthew J.; Willershausen, Ines; Barbeck, Mike; Balic, Ela; Görlach, Christoph; Stupp, Samuel I; Sader, Robert A.; Kirkpatrick, C. James.

In: Biomedical Materials, Vol. 6, No. 1, 015010, 2011.

Research output: Contribution to journalArticle

Ghanaati, S, Schlee, M, Webber, MJ, Willershausen, I, Barbeck, M, Balic, E, Görlach, C, Stupp, SI, Sader, RA & Kirkpatrick, CJ 2011, 'Evaluation of the tissue reaction to a new bilayered collagen matrix in vivo and its translation to the clinic', Biomedical Materials, vol. 6, no. 1, 015010. https://doi.org/10.1088/1748-6041/6/1/015010
Ghanaati, Shahram ; Schlee, Markus ; Webber, Matthew J. ; Willershausen, Ines ; Barbeck, Mike ; Balic, Ela ; Görlach, Christoph ; Stupp, Samuel I ; Sader, Robert A. ; Kirkpatrick, C. James. / Evaluation of the tissue reaction to a new bilayered collagen matrix in vivo and its translation to the clinic. In: Biomedical Materials. 2011 ; Vol. 6, No. 1.
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