Evidence for the absence of photoreduction of the metal centers of cytochrome C oxidase by X-irradiation.

Gary W Brudvig, D. F. Bocian, R. C. Gamble, S. I. Chan

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Samples of X-irradiated cytochrome c oxidase were examined by electron paramagnetic resonance and optical spectroscopy. Both radiation from the Stanford Synchrotron Radiation Laboratory and a conventional X-ray source (W target) were utilized. The X-ray flux from these sources ranges from 10(9) to 10(13) photons/s. No evidence was found for photoreduction of the metal centers in the enzyme by X-ray photons. These results demonstrate that the integrity of cytochrome c oxidase is maintained using the conditions under which X-ray absorption measurements are presently being made.

Original languageEnglish
Pages (from-to)78-89
Number of pages12
JournalBiochimica et biophysica acta
Issue number1
Publication statusPublished - Jul 24 1980


ASJC Scopus subject areas

  • Medicine(all)

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