Evolutionary significance of an algal gene encoding an [FeFe]-hydrogenase with F-domain homology and hydrogenase activity in Chlorella variabilis NC64A

Jonathan E. Meuser, Eric S. Boyd, Gennady Ananyev, Devin Karns, Randor Radakovits, U. M Narayana Murthy, Maria L. Ghirardi, G Charles Dismukes, John W. Peters, Matthew C. Posewitz

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

[FeFe]-hydrogenases (HYDA) link the production of molecular H2 to anaerobic metabolism in many green algae. Similar to Chlamydomonas reinhardtii, Chlorella variabilis NC64A (Trebouxiophyceae, Chlorophyta) exhibits [FeFe]-hydrogenase (HYDA) activity during anoxia. In contrast to C. reinhardtii and other chlorophycean algae, which contain hydrogenases with only the HYDA active site (H-cluster), C. variabilis NC64A is the only known green alga containing HYDA genes encoding accessory FeS cluster-binding domains (F-cluster). cDNA sequencing confirmed the presence of F-cluster HYDA1 mRNA transcripts, and identified deviations from the in silico splicing models. We show that HYDA activity in C. variabilis NC64A is coupled to anoxic photosynthetic electron transport (PSII linked, as well as PSII-independent) and dark fermentation. We also show that the in vivo H2-photoproduction activity observed is as O2 sensitive as in C. reinhardtii. The two C. variabilis NC64A HYDA sequences are similar to homologs found in more deeply branching bacteria (Thermotogales), diatoms, and heterotrophic flagellates, suggesting that an F-cluster HYDA is the ancestral enzyme in algae. Phylogenetic analysis indicates that the algal HYDA H-cluster domains are monophyletic, suggesting that they share a common origin, and evolved from a single ancestral F-cluster HYDA. Furthermore, phylogenetic reconstruction indicates that the multiple algal HYDA paralogs are the result of gene duplication events that occurred independently within each algal lineage. Collectively, comparative genomic, physiological, and phylogenetic analyses of the C. variabilis NC64A hydrogenase has provided new insights into the molecular evolution and diversity of algal [FeFe]-hydrogenases.

Original languageEnglish
Pages (from-to)829-843
Number of pages15
JournalPlanta
Volume234
Issue number4
DOIs
Publication statusPublished - Oct 2011

Fingerprint

ferredoxin hydrogenase
Chlorella
Hydrogenase
sequence homology
Genes
genes
Chlamydomonas reinhardtii
algae
Chlorophyta
phylogeny
Thermotogales
Anaerobiosis
Diatoms
hydrogen production
Gene Duplication
Molecular Evolution
Bacillariophyceae
anaerobiosis
gene duplication
Electron Transport

Keywords

  • [FeFe]-hydrogenase
  • Chlamydomonas reinhardtii
  • Chlorella variabilis NC64A
  • F-cluster domain
  • HYDA
  • Trebouxiophyceae

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

Meuser, J. E., Boyd, E. S., Ananyev, G., Karns, D., Radakovits, R., Murthy, U. M. N., ... Posewitz, M. C. (2011). Evolutionary significance of an algal gene encoding an [FeFe]-hydrogenase with F-domain homology and hydrogenase activity in Chlorella variabilis NC64A. Planta, 234(4), 829-843. https://doi.org/10.1007/s00425-011-1431-y

Evolutionary significance of an algal gene encoding an [FeFe]-hydrogenase with F-domain homology and hydrogenase activity in Chlorella variabilis NC64A. / Meuser, Jonathan E.; Boyd, Eric S.; Ananyev, Gennady; Karns, Devin; Radakovits, Randor; Murthy, U. M Narayana; Ghirardi, Maria L.; Dismukes, G Charles; Peters, John W.; Posewitz, Matthew C.

In: Planta, Vol. 234, No. 4, 10.2011, p. 829-843.

Research output: Contribution to journalArticle

Meuser, JE, Boyd, ES, Ananyev, G, Karns, D, Radakovits, R, Murthy, UMN, Ghirardi, ML, Dismukes, GC, Peters, JW & Posewitz, MC 2011, 'Evolutionary significance of an algal gene encoding an [FeFe]-hydrogenase with F-domain homology and hydrogenase activity in Chlorella variabilis NC64A', Planta, vol. 234, no. 4, pp. 829-843. https://doi.org/10.1007/s00425-011-1431-y
Meuser, Jonathan E. ; Boyd, Eric S. ; Ananyev, Gennady ; Karns, Devin ; Radakovits, Randor ; Murthy, U. M Narayana ; Ghirardi, Maria L. ; Dismukes, G Charles ; Peters, John W. ; Posewitz, Matthew C. / Evolutionary significance of an algal gene encoding an [FeFe]-hydrogenase with F-domain homology and hydrogenase activity in Chlorella variabilis NC64A. In: Planta. 2011 ; Vol. 234, No. 4. pp. 829-843.
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abstract = "[FeFe]-hydrogenases (HYDA) link the production of molecular H2 to anaerobic metabolism in many green algae. Similar to Chlamydomonas reinhardtii, Chlorella variabilis NC64A (Trebouxiophyceae, Chlorophyta) exhibits [FeFe]-hydrogenase (HYDA) activity during anoxia. In contrast to C. reinhardtii and other chlorophycean algae, which contain hydrogenases with only the HYDA active site (H-cluster), C. variabilis NC64A is the only known green alga containing HYDA genes encoding accessory FeS cluster-binding domains (F-cluster). cDNA sequencing confirmed the presence of F-cluster HYDA1 mRNA transcripts, and identified deviations from the in silico splicing models. We show that HYDA activity in C. variabilis NC64A is coupled to anoxic photosynthetic electron transport (PSII linked, as well as PSII-independent) and dark fermentation. We also show that the in vivo H2-photoproduction activity observed is as O2 sensitive as in C. reinhardtii. The two C. variabilis NC64A HYDA sequences are similar to homologs found in more deeply branching bacteria (Thermotogales), diatoms, and heterotrophic flagellates, suggesting that an F-cluster HYDA is the ancestral enzyme in algae. Phylogenetic analysis indicates that the algal HYDA H-cluster domains are monophyletic, suggesting that they share a common origin, and evolved from a single ancestral F-cluster HYDA. Furthermore, phylogenetic reconstruction indicates that the multiple algal HYDA paralogs are the result of gene duplication events that occurred independently within each algal lineage. Collectively, comparative genomic, physiological, and phylogenetic analyses of the C. variabilis NC64A hydrogenase has provided new insights into the molecular evolution and diversity of algal [FeFe]-hydrogenases.",
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