Excitation energy transfer and charge separation in the isolated Photosystem II reaction center

Scott R. Greenfield, Michael R Wasielewski

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The nature of excitation energy transfer and charge separation in isolated Photosystem II reaction centers is an area of considerable interest and controversy. Excitation energy transfer from accessory chlorophyll a to the primary electron donor P680 takes place in tens of picoseconds, although there is some evidence that thermal equilibration of the excitation between P680 and a subset of the accessory chlorophyll a occurs on a 100-fs timescale. The intrinsic rate for charge separation at low temperature is accepted to be ca. (2 ps)-1, and is based on several measurements using different experimental techniques. This rate is in good agreement with estimates based on larger sized particles, and is similar to the rate observed with bacterial reaction centers. However, near room temperature there is considerable disagreement as to the observed rate for charge separation, with several experiments pointing to a ca. (3 ps)-1 rate, and others to a ca. (20 ps)-1 rate. These processes and the experiments used to measure them will be reviewed.

Original languageEnglish
Pages (from-to)83-97
Number of pages15
JournalPhotosynthesis Research
Volume48
Issue number1-2
Publication statusPublished - 1996

Fingerprint

Photosystem II Protein Complex
Excitation energy
Energy Transfer
energy transfer
Energy transfer
photosystem II
Accessories
Temperature
chlorophyll
Hot Temperature
Electrons
ambient temperature
Experiments
electrons
heat
chlorophyll a
temperature
methodology

Keywords

  • electron transfer
  • P680
  • pheophytin
  • primary photochemistry
  • ultrafast spectroscopy

ASJC Scopus subject areas

  • Plant Science

Cite this

Excitation energy transfer and charge separation in the isolated Photosystem II reaction center. / Greenfield, Scott R.; Wasielewski, Michael R.

In: Photosynthesis Research, Vol. 48, No. 1-2, 1996, p. 83-97.

Research output: Contribution to journalArticle

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