Fluorescence antibunching microscopy

Osip Schwartz, Jonathan M. Levitt, Dan Oron

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Utilizing quantum properties of light to break the diffraction limit has been the goal of intense research in the recent years. This paper is a progress report on a study aimed at experimentally demonstrating a superresolution microscopy technique enabled by photon antibunching, a non-classical emission statistics feature exhibited by most emitters used as fluorescent markers. We find that photon antibunching gives rise to correlations that encode high spatial frequency information on the distribution of fluorescent emitters. Detecting these correlations using photon counting instrumentation in a standard fluorescence microscope setting allows for three-dimensional superresolution imaging of fluorophore stained samples. The technique provides a quantum alternative to the established superresolution tools.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8228
DOIs
Publication statusPublished - 2012
EventSingle Molecule Spectroscopy and Superresolution Imaging V - San Francisco, CA, United States
Duration: Jan 21 2012Jan 22 2012

Other

OtherSingle Molecule Spectroscopy and Superresolution Imaging V
CountryUnited States
CitySan Francisco, CA
Period1/21/121/22/12

Fingerprint

Fluorescence microscopy
Photons
Fluorescence Microscopy
microscopy
fluorescence
emitters
photons
Three-Dimensional Imaging
Information Dissemination
Fluorophores
markers
Microscopy
Microscopic examination
counting
Microscopes
Diffraction
Fluorescence
microscopes
Statistics
statistics

Keywords

  • fluorescence microscopy
  • quantum imaging
  • superresolution

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Schwartz, O., Levitt, J. M., & Oron, D. (2012). Fluorescence antibunching microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8228). [822802] https://doi.org/10.1117/12.907807

Fluorescence antibunching microscopy. / Schwartz, Osip; Levitt, Jonathan M.; Oron, Dan.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8228 2012. 822802.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Schwartz, O, Levitt, JM & Oron, D 2012, Fluorescence antibunching microscopy. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8228, 822802, Single Molecule Spectroscopy and Superresolution Imaging V, San Francisco, CA, United States, 1/21/12. https://doi.org/10.1117/12.907807
Schwartz O, Levitt JM, Oron D. Fluorescence antibunching microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8228. 2012. 822802 https://doi.org/10.1117/12.907807
Schwartz, Osip ; Levitt, Jonathan M. ; Oron, Dan. / Fluorescence antibunching microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8228 2012.
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