Fluorescence antibunching microscopy

Osip Schwartz, Jonathan M. Levitt, Dan Oron

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Utilizing quantum properties of light to break the diffraction limit has been the goal of intense research in the recent years. This paper is a progress report on a study aimed at experimentally demonstrating a superresolution microscopy technique enabled by photon antibunching, a non-classical emission statistics feature exhibited by most emitters used as fluorescent markers. We find that photon antibunching gives rise to correlations that encode high spatial frequency information on the distribution of fluorescent emitters. Detecting these correlations using photon counting instrumentation in a standard fluorescence microscope setting allows for three-dimensional superresolution imaging of fluorophore stained samples. The technique provides a quantum alternative to the established superresolution tools.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging V
DOIs
Publication statusPublished - Mar 5 2012
EventSingle Molecule Spectroscopy and Superresolution Imaging V - San Francisco, CA, United States
Duration: Jan 21 2012Jan 22 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8228
ISSN (Print)1605-7422

Other

OtherSingle Molecule Spectroscopy and Superresolution Imaging V
CountryUnited States
CitySan Francisco, CA
Period1/21/121/22/12

Keywords

  • fluorescence microscopy
  • quantum imaging
  • superresolution

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Schwartz, O., Levitt, J. M., & Oron, D. (2012). Fluorescence antibunching microscopy. In Single Molecule Spectroscopy and Superresolution Imaging V [822802] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8228). https://doi.org/10.1117/12.907807