Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy

Noël Boens, Wenwu Qin, Nikola Basarić, Johan Hofkens, Marcel Ameloot, Jacques Pouget, Jean Pierre Lefèvre, Bernard Valeur, Enrico Gratton, Martin VandeVen, Norberto D. Silva, Yves Engelborghs, Katrien Willaert, Alain Sillen, Gary Rumbles, David Phillips, Antonie J W G Visser, Arie Van Hoek, Joseph R. Lakowicz, Henryk Malak & 5 others Ignacy Gryczynski, Arthur G. Szabo, Don T. Krajcarski, Naoto Tamai, Atsushi Miura

Research output: Contribution to journalArticle

265 Citations (Scopus)

Abstract

A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 °C were measured independently by nine laboratories using single-photon timing and multifrequency phase and modulation fluorometry instruments with lasers as excitation source. The dyes that can serve as fluorescence lifetime standards for time-domain and frequency-domain measurements are all commercially available, are photostable under the conditions of the measurements, and are soluble in solvents of spectroscopic quality (methanol, cyclohexane, water). These lifetime standards are anthracene, 9-cyanoanthracene, 9,10-diphenylanthracene, N-methylcarbazole, coumarin 153, erythrosin B, N-acetyl-L-tryptophanamide, 1,4-bis(5-phenyloxazol-2-yl)benzene, 2,5-diphenyloxazole, rhodamine B, rubrene, AT-(3-sulfopropyl)acridinium, and 1,4-diphenylbenzene. At 20 °C, the fluorescence lifetimes vary from 89 ps to 31.2 ns, depending on fluorescent dye and solvent, which is a useful range for modern pico- and nanosecond time-domain or mega- to gigahertz frequency-domain instrumentation. The decay times are independent of the excitation and emission wavelengths. Dependent on the structure of the dye and the solvent, the excitation wavelengths used range from 284 to 575 nm, the emission from 330 to 630 nm. These lifetime standards may be used to either calibrate or test the resolution of time- and frequency-domain instrumentation or as reference compounds to eliminate the color effect in photomultiplier tubes. Statistical analyses by means of two-sample charts indicate that there is no laboratory bias in the lifetime determinations. Moreover, statistical tests show that there is an excellent correlation between the lifetimes estimated by the time-domain and frequency-domain fluorometries. Comprehensive tables compiling the results for 20 (fluorescence lifetime standard/solvent) combinations are given.

Original languageEnglish
Pages (from-to)2137-2149
Number of pages13
JournalAnalytical Chemistry
Volume79
Issue number5
DOIs
Publication statusPublished - Mar 5 2007

Fingerprint

Fluorescence spectroscopy
Fluorescence
rhodamine B
Coloring Agents
Erythrosine
Wavelength
Fluorophores
Statistical tests
Photomultipliers
Benzene
Fluorescent Dyes
Methanol
Light sources
Photons
Modulation
Color
Water
Lasers
Liquids

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Boens, N., Qin, W., Basarić, N., Hofkens, J., Ameloot, M., Pouget, J., ... Miura, A. (2007). Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy. Analytical Chemistry, 79(5), 2137-2149. https://doi.org/10.1021/ac062160k

Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy. / Boens, Noël; Qin, Wenwu; Basarić, Nikola; Hofkens, Johan; Ameloot, Marcel; Pouget, Jacques; Lefèvre, Jean Pierre; Valeur, Bernard; Gratton, Enrico; VandeVen, Martin; Silva, Norberto D.; Engelborghs, Yves; Willaert, Katrien; Sillen, Alain; Rumbles, Gary; Phillips, David; Visser, Antonie J W G; Van Hoek, Arie; Lakowicz, Joseph R.; Malak, Henryk; Gryczynski, Ignacy; Szabo, Arthur G.; Krajcarski, Don T.; Tamai, Naoto; Miura, Atsushi.

In: Analytical Chemistry, Vol. 79, No. 5, 05.03.2007, p. 2137-2149.

Research output: Contribution to journalArticle

Boens, N, Qin, W, Basarić, N, Hofkens, J, Ameloot, M, Pouget, J, Lefèvre, JP, Valeur, B, Gratton, E, VandeVen, M, Silva, ND, Engelborghs, Y, Willaert, K, Sillen, A, Rumbles, G, Phillips, D, Visser, AJWG, Van Hoek, A, Lakowicz, JR, Malak, H, Gryczynski, I, Szabo, AG, Krajcarski, DT, Tamai, N & Miura, A 2007, 'Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy', Analytical Chemistry, vol. 79, no. 5, pp. 2137-2149. https://doi.org/10.1021/ac062160k
Boens N, Qin W, Basarić N, Hofkens J, Ameloot M, Pouget J et al. Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy. Analytical Chemistry. 2007 Mar 5;79(5):2137-2149. https://doi.org/10.1021/ac062160k
Boens, Noël ; Qin, Wenwu ; Basarić, Nikola ; Hofkens, Johan ; Ameloot, Marcel ; Pouget, Jacques ; Lefèvre, Jean Pierre ; Valeur, Bernard ; Gratton, Enrico ; VandeVen, Martin ; Silva, Norberto D. ; Engelborghs, Yves ; Willaert, Katrien ; Sillen, Alain ; Rumbles, Gary ; Phillips, David ; Visser, Antonie J W G ; Van Hoek, Arie ; Lakowicz, Joseph R. ; Malak, Henryk ; Gryczynski, Ignacy ; Szabo, Arthur G. ; Krajcarski, Don T. ; Tamai, Naoto ; Miura, Atsushi. / Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy. In: Analytical Chemistry. 2007 ; Vol. 79, No. 5. pp. 2137-2149.
@article{499bc92072af4044875b951722662b9f,
title = "Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy",
abstract = "A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 °C were measured independently by nine laboratories using single-photon timing and multifrequency phase and modulation fluorometry instruments with lasers as excitation source. The dyes that can serve as fluorescence lifetime standards for time-domain and frequency-domain measurements are all commercially available, are photostable under the conditions of the measurements, and are soluble in solvents of spectroscopic quality (methanol, cyclohexane, water). These lifetime standards are anthracene, 9-cyanoanthracene, 9,10-diphenylanthracene, N-methylcarbazole, coumarin 153, erythrosin B, N-acetyl-L-tryptophanamide, 1,4-bis(5-phenyloxazol-2-yl)benzene, 2,5-diphenyloxazole, rhodamine B, rubrene, AT-(3-sulfopropyl)acridinium, and 1,4-diphenylbenzene. At 20 °C, the fluorescence lifetimes vary from 89 ps to 31.2 ns, depending on fluorescent dye and solvent, which is a useful range for modern pico- and nanosecond time-domain or mega- to gigahertz frequency-domain instrumentation. The decay times are independent of the excitation and emission wavelengths. Dependent on the structure of the dye and the solvent, the excitation wavelengths used range from 284 to 575 nm, the emission from 330 to 630 nm. These lifetime standards may be used to either calibrate or test the resolution of time- and frequency-domain instrumentation or as reference compounds to eliminate the color effect in photomultiplier tubes. Statistical analyses by means of two-sample charts indicate that there is no laboratory bias in the lifetime determinations. Moreover, statistical tests show that there is an excellent correlation between the lifetimes estimated by the time-domain and frequency-domain fluorometries. Comprehensive tables compiling the results for 20 (fluorescence lifetime standard/solvent) combinations are given.",
author = "No{\"e}l Boens and Wenwu Qin and Nikola Basarić and Johan Hofkens and Marcel Ameloot and Jacques Pouget and Lef{\`e}vre, {Jean Pierre} and Bernard Valeur and Enrico Gratton and Martin VandeVen and Silva, {Norberto D.} and Yves Engelborghs and Katrien Willaert and Alain Sillen and Gary Rumbles and David Phillips and Visser, {Antonie J W G} and {Van Hoek}, Arie and Lakowicz, {Joseph R.} and Henryk Malak and Ignacy Gryczynski and Szabo, {Arthur G.} and Krajcarski, {Don T.} and Naoto Tamai and Atsushi Miura",
year = "2007",
month = "3",
day = "5",
doi = "10.1021/ac062160k",
language = "English",
volume = "79",
pages = "2137--2149",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "5",

}

TY - JOUR

T1 - Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy

AU - Boens, Noël

AU - Qin, Wenwu

AU - Basarić, Nikola

AU - Hofkens, Johan

AU - Ameloot, Marcel

AU - Pouget, Jacques

AU - Lefèvre, Jean Pierre

AU - Valeur, Bernard

AU - Gratton, Enrico

AU - VandeVen, Martin

AU - Silva, Norberto D.

AU - Engelborghs, Yves

AU - Willaert, Katrien

AU - Sillen, Alain

AU - Rumbles, Gary

AU - Phillips, David

AU - Visser, Antonie J W G

AU - Van Hoek, Arie

AU - Lakowicz, Joseph R.

AU - Malak, Henryk

AU - Gryczynski, Ignacy

AU - Szabo, Arthur G.

AU - Krajcarski, Don T.

AU - Tamai, Naoto

AU - Miura, Atsushi

PY - 2007/3/5

Y1 - 2007/3/5

N2 - A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 °C were measured independently by nine laboratories using single-photon timing and multifrequency phase and modulation fluorometry instruments with lasers as excitation source. The dyes that can serve as fluorescence lifetime standards for time-domain and frequency-domain measurements are all commercially available, are photostable under the conditions of the measurements, and are soluble in solvents of spectroscopic quality (methanol, cyclohexane, water). These lifetime standards are anthracene, 9-cyanoanthracene, 9,10-diphenylanthracene, N-methylcarbazole, coumarin 153, erythrosin B, N-acetyl-L-tryptophanamide, 1,4-bis(5-phenyloxazol-2-yl)benzene, 2,5-diphenyloxazole, rhodamine B, rubrene, AT-(3-sulfopropyl)acridinium, and 1,4-diphenylbenzene. At 20 °C, the fluorescence lifetimes vary from 89 ps to 31.2 ns, depending on fluorescent dye and solvent, which is a useful range for modern pico- and nanosecond time-domain or mega- to gigahertz frequency-domain instrumentation. The decay times are independent of the excitation and emission wavelengths. Dependent on the structure of the dye and the solvent, the excitation wavelengths used range from 284 to 575 nm, the emission from 330 to 630 nm. These lifetime standards may be used to either calibrate or test the resolution of time- and frequency-domain instrumentation or as reference compounds to eliminate the color effect in photomultiplier tubes. Statistical analyses by means of two-sample charts indicate that there is no laboratory bias in the lifetime determinations. Moreover, statistical tests show that there is an excellent correlation between the lifetimes estimated by the time-domain and frequency-domain fluorometries. Comprehensive tables compiling the results for 20 (fluorescence lifetime standard/solvent) combinations are given.

AB - A series of fluorophores with single-exponential fluorescence decays in liquid solution at 20 °C were measured independently by nine laboratories using single-photon timing and multifrequency phase and modulation fluorometry instruments with lasers as excitation source. The dyes that can serve as fluorescence lifetime standards for time-domain and frequency-domain measurements are all commercially available, are photostable under the conditions of the measurements, and are soluble in solvents of spectroscopic quality (methanol, cyclohexane, water). These lifetime standards are anthracene, 9-cyanoanthracene, 9,10-diphenylanthracene, N-methylcarbazole, coumarin 153, erythrosin B, N-acetyl-L-tryptophanamide, 1,4-bis(5-phenyloxazol-2-yl)benzene, 2,5-diphenyloxazole, rhodamine B, rubrene, AT-(3-sulfopropyl)acridinium, and 1,4-diphenylbenzene. At 20 °C, the fluorescence lifetimes vary from 89 ps to 31.2 ns, depending on fluorescent dye and solvent, which is a useful range for modern pico- and nanosecond time-domain or mega- to gigahertz frequency-domain instrumentation. The decay times are independent of the excitation and emission wavelengths. Dependent on the structure of the dye and the solvent, the excitation wavelengths used range from 284 to 575 nm, the emission from 330 to 630 nm. These lifetime standards may be used to either calibrate or test the resolution of time- and frequency-domain instrumentation or as reference compounds to eliminate the color effect in photomultiplier tubes. Statistical analyses by means of two-sample charts indicate that there is no laboratory bias in the lifetime determinations. Moreover, statistical tests show that there is an excellent correlation between the lifetimes estimated by the time-domain and frequency-domain fluorometries. Comprehensive tables compiling the results for 20 (fluorescence lifetime standard/solvent) combinations are given.

UR - http://www.scopus.com/inward/record.url?scp=33847620267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847620267&partnerID=8YFLogxK

U2 - 10.1021/ac062160k

DO - 10.1021/ac062160k

M3 - Article

VL - 79

SP - 2137

EP - 2149

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 5

ER -