Functional patterned multiphoton excitation deep inside scattering tissue

Eirini Papagiakoumou, Aurélien Bègue, Ben Leshem, Osip Schwartz, Brandon M. Stell, Jonathan Bradley, Dan Oron, Valentina Emiliani

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Stochastic distortion of light beams in scattering samples makes in-depth photoexcitation in brain tissue a major challenge. A common solution for overcoming scattering involves adaptive pre-compensation of the unknown distortion1-3. However, this requires long iterative searches for sample-specific optimized corrections, which is a problem when applied to optical neurostimulation where typical timescales in the system are in the millisecond range. Thus, photoexcitation in scattering media that is independent of the properties of a specific sample would be an ideal solution. Here, we show that temporally focused two-photon excitation4with generalized phase contrast5 enables photoexcitation of arbitrary spatial patterns within turbid tissues with remarkable robustness to scattering. We demonstrate three-dimensional confinement of tailored photoexcitation patterns >200 μm in depth, both in numerical simulations and through brain slices combined with patch-clamp recording of photoactivated channelrhodopsin-2.

Original languageEnglish
Pages (from-to)274-278
Number of pages5
JournalNature Photonics
Volume7
Issue number4
DOIs
Publication statusPublished - Apr 2013

Fingerprint

Photoexcitation
photoexcitation
Scattering
Tissue
scattering
excitation
brain
Brain
clamps
Clamping devices
light beams
Photons
recording
Computer simulation
photons
simulation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Papagiakoumou, E., Bègue, A., Leshem, B., Schwartz, O., Stell, B. M., Bradley, J., ... Emiliani, V. (2013). Functional patterned multiphoton excitation deep inside scattering tissue. Nature Photonics, 7(4), 274-278. https://doi.org/10.1038/nphoton.2013.9

Functional patterned multiphoton excitation deep inside scattering tissue. / Papagiakoumou, Eirini; Bègue, Aurélien; Leshem, Ben; Schwartz, Osip; Stell, Brandon M.; Bradley, Jonathan; Oron, Dan; Emiliani, Valentina.

In: Nature Photonics, Vol. 7, No. 4, 04.2013, p. 274-278.

Research output: Contribution to journalArticle

Papagiakoumou, E, Bègue, A, Leshem, B, Schwartz, O, Stell, BM, Bradley, J, Oron, D & Emiliani, V 2013, 'Functional patterned multiphoton excitation deep inside scattering tissue', Nature Photonics, vol. 7, no. 4, pp. 274-278. https://doi.org/10.1038/nphoton.2013.9
Papagiakoumou E, Bègue A, Leshem B, Schwartz O, Stell BM, Bradley J et al. Functional patterned multiphoton excitation deep inside scattering tissue. Nature Photonics. 2013 Apr;7(4):274-278. https://doi.org/10.1038/nphoton.2013.9
Papagiakoumou, Eirini ; Bègue, Aurélien ; Leshem, Ben ; Schwartz, Osip ; Stell, Brandon M. ; Bradley, Jonathan ; Oron, Dan ; Emiliani, Valentina. / Functional patterned multiphoton excitation deep inside scattering tissue. In: Nature Photonics. 2013 ; Vol. 7, No. 4. pp. 274-278.
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