TY - JOUR
T1 - Functional patterned multiphoton excitation deep inside scattering tissue
AU - Papagiakoumou, Eirini
AU - Bègue, Aurélien
AU - Leshem, Ben
AU - Schwartz, Osip
AU - Stell, Brandon M.
AU - Bradley, Jonathan
AU - Oron, Dan
AU - Emiliani, Valentina
N1 - Funding Information:
The authors thank J. Montanaro, G. Bouchery and A. Schorscher-Petcu for the preparation of fixed slices, M. Briand for her contribution to data analysis and A. Teitelboim for her help in preparing scattering liquid solutions. E.P. was supported by the Fondation pour la Recherche Médicale (FRM). A.B. was supported by the Ecole des Neurosciences de Paris (ENP). V.E. was supported by the Human Frontier Science Program (RGP0013/2010) and the Fondation pour la Recherche Médicale (FRM équipe). D.O. acknowledges support by a European Research Council starting investigator grant (no. SINSLIM 258221) and the Crown Center of Photonics. O.S. is supported by the Adams Fellowships programme of the Israel Academy of Sciences and Humanities. The Emiliani group would like to thank A. Marty and I. Llano for providing space to host the optical set-up during the renovation works in the laboratory and D. Ogden for providing scientific equipment for the electrophysiology setup.
PY - 2013/4
Y1 - 2013/4
N2 - Stochastic distortion of light beams in scattering samples makes in-depth photoexcitation in brain tissue a major challenge. A common solution for overcoming scattering involves adaptive pre-compensation of the unknown distortion1-3. However, this requires long iterative searches for sample-specific optimized corrections, which is a problem when applied to optical neurostimulation where typical timescales in the system are in the millisecond range. Thus, photoexcitation in scattering media that is independent of the properties of a specific sample would be an ideal solution. Here, we show that temporally focused two-photon excitation4with generalized phase contrast5 enables photoexcitation of arbitrary spatial patterns within turbid tissues with remarkable robustness to scattering. We demonstrate three-dimensional confinement of tailored photoexcitation patterns >200 μm in depth, both in numerical simulations and through brain slices combined with patch-clamp recording of photoactivated channelrhodopsin-2.
AB - Stochastic distortion of light beams in scattering samples makes in-depth photoexcitation in brain tissue a major challenge. A common solution for overcoming scattering involves adaptive pre-compensation of the unknown distortion1-3. However, this requires long iterative searches for sample-specific optimized corrections, which is a problem when applied to optical neurostimulation where typical timescales in the system are in the millisecond range. Thus, photoexcitation in scattering media that is independent of the properties of a specific sample would be an ideal solution. Here, we show that temporally focused two-photon excitation4with generalized phase contrast5 enables photoexcitation of arbitrary spatial patterns within turbid tissues with remarkable robustness to scattering. We demonstrate three-dimensional confinement of tailored photoexcitation patterns >200 μm in depth, both in numerical simulations and through brain slices combined with patch-clamp recording of photoactivated channelrhodopsin-2.
UR - http://www.scopus.com/inward/record.url?scp=84875812759&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875812759&partnerID=8YFLogxK
U2 - 10.1038/nphoton.2013.9
DO - 10.1038/nphoton.2013.9
M3 - Article
AN - SCOPUS:84875812759
VL - 7
SP - 274
EP - 278
JO - Nature Photonics
JF - Nature Photonics
SN - 1749-4885
IS - 4
ER -