Functional patterned multiphoton excitation deep inside scattering tissue

Eirini Papagiakoumou, Aurélien Bègue, Ben Leshem, Osip Schwartz, Brandon M. Stell, Jonathan Bradley, Dan Oron, Valentina Emiliani

Research output: Contribution to journalArticlepeer-review

85 Citations (Scopus)


Stochastic distortion of light beams in scattering samples makes in-depth photoexcitation in brain tissue a major challenge. A common solution for overcoming scattering involves adaptive pre-compensation of the unknown distortion1-3. However, this requires long iterative searches for sample-specific optimized corrections, which is a problem when applied to optical neurostimulation where typical timescales in the system are in the millisecond range. Thus, photoexcitation in scattering media that is independent of the properties of a specific sample would be an ideal solution. Here, we show that temporally focused two-photon excitation4with generalized phase contrast5 enables photoexcitation of arbitrary spatial patterns within turbid tissues with remarkable robustness to scattering. We demonstrate three-dimensional confinement of tailored photoexcitation patterns >200 μm in depth, both in numerical simulations and through brain slices combined with patch-clamp recording of photoactivated channelrhodopsin-2.

Original languageEnglish
Pages (from-to)274-278
Number of pages5
JournalNature Photonics
Issue number4
Publication statusPublished - Apr 2013

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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