Abstract
Stochastic distortion of light beams in scattering samples makes in-depth photoexcitation in brain tissue a major challenge. A common solution for overcoming scattering involves adaptive pre-compensation of the unknown distortion1-3. However, this requires long iterative searches for sample-specific optimized corrections, which is a problem when applied to optical neurostimulation where typical timescales in the system are in the millisecond range. Thus, photoexcitation in scattering media that is independent of the properties of a specific sample would be an ideal solution. Here, we show that temporally focused two-photon excitation4with generalized phase contrast5 enables photoexcitation of arbitrary spatial patterns within turbid tissues with remarkable robustness to scattering. We demonstrate three-dimensional confinement of tailored photoexcitation patterns >200 μm in depth, both in numerical simulations and through brain slices combined with patch-clamp recording of photoactivated channelrhodopsin-2.
| Original language | English |
|---|---|
| Pages (from-to) | 274-278 |
| Number of pages | 5 |
| Journal | Nature Photonics |
| Volume | 7 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 2013 |
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ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
Cite this
Functional patterned multiphoton excitation deep inside scattering tissue. / Papagiakoumou, Eirini; Bègue, Aurélien; Leshem, Ben; Schwartz, Osip; Stell, Brandon M.; Bradley, Jonathan; Oron, Dan; Emiliani, Valentina.
In: Nature Photonics, Vol. 7, No. 4, 04.2013, p. 274-278.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional patterned multiphoton excitation deep inside scattering tissue
AU - Papagiakoumou, Eirini
AU - Bègue, Aurélien
AU - Leshem, Ben
AU - Schwartz, Osip
AU - Stell, Brandon M.
AU - Bradley, Jonathan
AU - Oron, Dan
AU - Emiliani, Valentina
PY - 2013/4
Y1 - 2013/4
N2 - Stochastic distortion of light beams in scattering samples makes in-depth photoexcitation in brain tissue a major challenge. A common solution for overcoming scattering involves adaptive pre-compensation of the unknown distortion1-3. However, this requires long iterative searches for sample-specific optimized corrections, which is a problem when applied to optical neurostimulation where typical timescales in the system are in the millisecond range. Thus, photoexcitation in scattering media that is independent of the properties of a specific sample would be an ideal solution. Here, we show that temporally focused two-photon excitation4with generalized phase contrast5 enables photoexcitation of arbitrary spatial patterns within turbid tissues with remarkable robustness to scattering. We demonstrate three-dimensional confinement of tailored photoexcitation patterns >200 μm in depth, both in numerical simulations and through brain slices combined with patch-clamp recording of photoactivated channelrhodopsin-2.
AB - Stochastic distortion of light beams in scattering samples makes in-depth photoexcitation in brain tissue a major challenge. A common solution for overcoming scattering involves adaptive pre-compensation of the unknown distortion1-3. However, this requires long iterative searches for sample-specific optimized corrections, which is a problem when applied to optical neurostimulation where typical timescales in the system are in the millisecond range. Thus, photoexcitation in scattering media that is independent of the properties of a specific sample would be an ideal solution. Here, we show that temporally focused two-photon excitation4with generalized phase contrast5 enables photoexcitation of arbitrary spatial patterns within turbid tissues with remarkable robustness to scattering. We demonstrate three-dimensional confinement of tailored photoexcitation patterns >200 μm in depth, both in numerical simulations and through brain slices combined with patch-clamp recording of photoactivated channelrhodopsin-2.
UR - http://www.scopus.com/inward/record.url?scp=84875812759&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875812759&partnerID=8YFLogxK
U2 - 10.1038/nphoton.2013.9
DO - 10.1038/nphoton.2013.9
M3 - Article
AN - SCOPUS:84875812759
VL - 7
SP - 274
EP - 278
JO - Nature Photonics
JF - Nature Photonics
SN - 1749-4885
IS - 4
ER -