Gel Scaffolds of BMP-2-Binding Peptide Amphiphile Nanofibers for Spinal Arthrodesis

Sungsoo S. Lee, Erin L. Hsu, Marco Mendoza, Jason Ghodasra, Michael S. Nickoli, Amruta Ashtekar, Mahesh Polavarapu, Jacob Babu, Rehan M. Riaz, Joseph D. Nicolas, David Nelson, Sohaib Z. Hashmi, Stuart R. Kaltz, Jeffrey S. Earhart, Bradley R. Merk, Jeff S. Mckee, Shawn F. Bairstow, Ramille N. Shah, Wellington K. Hsu, Samuel I Stupp

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Peptide amphiphile (PA) nanofibers formed by self-assembly can be customized for specific applications in regenerative medicine through the use of molecules that display bioactive signals on their surfaces. Here, the use of PA nanofibers with binding affinity for the bone promoting growth factor BMP-2 to create a gel scaffold for osteogenesis is reported. With the objective of reducing the amount of BMP-2 used clinically for successful arthrodesis in the spine, amounts of growth factor incorporated in the scaffolds that are 10 to 100 times lower than that those used clinically in collagen scaffolds are used. The efficacy of the bioactive PA system to promote BMP-2-induced osteogenesis in vivo is investigated in a rat posterolateral lumbar intertransverse spinal fusion model. PA nanofiber gels displaying BMP-2-binding segments exhibit superior spinal fusion rates relative to controls, effectively decreasing the required therapeutic dose of BMP-2 by 10-fold. Interestingly, a 42% fusion rate is observed for gels containing the bioactive nanofibers without the use of exogenous BMP-2, suggesting the ability of the nanofiber to recruit endogenous growth factor. Results obtained here demonstrate that bioactive biomaterials with capacity to bind specific growth factors by design are great targets for regenerative medicine. Supramolecular nanofibers presenting BMP-2-binding epitopes on the surface exhibit superior spinal fusion rates in rats, effectively decreasing the therapeutic dose of BMP-2 by 10-fold. Importantly, the bioactive nanofibers elicit 42% fusion rate without the addition of exogenous BMP-2.

Original languageEnglish
Pages (from-to)131-141
Number of pages11
JournalAdvanced healthcare materials
Volume4
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Nanofibers
Amphiphiles
Arthrodesis
Scaffolds (biology)
Scaffolds
Peptides
Gels
Fusion reactions
Spinal Fusion
Intercellular Signaling Peptides and Proteins
Regenerative Medicine
Osteogenesis
Rats
Epitopes
Bone Development
Biocompatible Materials
Collagen
Biomaterials
Self assembly
Bone

Keywords

  • BMP-2 (bone morphogenetic protein-2)
  • Bone regeneration
  • Peptide amphiphile
  • Regenerative medicine
  • Spinal fusion

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Pharmaceutical Science

Cite this

Gel Scaffolds of BMP-2-Binding Peptide Amphiphile Nanofibers for Spinal Arthrodesis. / Lee, Sungsoo S.; Hsu, Erin L.; Mendoza, Marco; Ghodasra, Jason; Nickoli, Michael S.; Ashtekar, Amruta; Polavarapu, Mahesh; Babu, Jacob; Riaz, Rehan M.; Nicolas, Joseph D.; Nelson, David; Hashmi, Sohaib Z.; Kaltz, Stuart R.; Earhart, Jeffrey S.; Merk, Bradley R.; Mckee, Jeff S.; Bairstow, Shawn F.; Shah, Ramille N.; Hsu, Wellington K.; Stupp, Samuel I.

In: Advanced healthcare materials, Vol. 4, No. 1, 01.01.2015, p. 131-141.

Research output: Contribution to journalArticle

Lee, SS, Hsu, EL, Mendoza, M, Ghodasra, J, Nickoli, MS, Ashtekar, A, Polavarapu, M, Babu, J, Riaz, RM, Nicolas, JD, Nelson, D, Hashmi, SZ, Kaltz, SR, Earhart, JS, Merk, BR, Mckee, JS, Bairstow, SF, Shah, RN, Hsu, WK & Stupp, SI 2015, 'Gel Scaffolds of BMP-2-Binding Peptide Amphiphile Nanofibers for Spinal Arthrodesis', Advanced healthcare materials, vol. 4, no. 1, pp. 131-141. https://doi.org/10.1002/adhm.201400129
Lee, Sungsoo S. ; Hsu, Erin L. ; Mendoza, Marco ; Ghodasra, Jason ; Nickoli, Michael S. ; Ashtekar, Amruta ; Polavarapu, Mahesh ; Babu, Jacob ; Riaz, Rehan M. ; Nicolas, Joseph D. ; Nelson, David ; Hashmi, Sohaib Z. ; Kaltz, Stuart R. ; Earhart, Jeffrey S. ; Merk, Bradley R. ; Mckee, Jeff S. ; Bairstow, Shawn F. ; Shah, Ramille N. ; Hsu, Wellington K. ; Stupp, Samuel I. / Gel Scaffolds of BMP-2-Binding Peptide Amphiphile Nanofibers for Spinal Arthrodesis. In: Advanced healthcare materials. 2015 ; Vol. 4, No. 1. pp. 131-141.
@article{94f5ffdace8c4082be9616b6f8537d10,
title = "Gel Scaffolds of BMP-2-Binding Peptide Amphiphile Nanofibers for Spinal Arthrodesis",
abstract = "Peptide amphiphile (PA) nanofibers formed by self-assembly can be customized for specific applications in regenerative medicine through the use of molecules that display bioactive signals on their surfaces. Here, the use of PA nanofibers with binding affinity for the bone promoting growth factor BMP-2 to create a gel scaffold for osteogenesis is reported. With the objective of reducing the amount of BMP-2 used clinically for successful arthrodesis in the spine, amounts of growth factor incorporated in the scaffolds that are 10 to 100 times lower than that those used clinically in collagen scaffolds are used. The efficacy of the bioactive PA system to promote BMP-2-induced osteogenesis in vivo is investigated in a rat posterolateral lumbar intertransverse spinal fusion model. PA nanofiber gels displaying BMP-2-binding segments exhibit superior spinal fusion rates relative to controls, effectively decreasing the required therapeutic dose of BMP-2 by 10-fold. Interestingly, a 42{\%} fusion rate is observed for gels containing the bioactive nanofibers without the use of exogenous BMP-2, suggesting the ability of the nanofiber to recruit endogenous growth factor. Results obtained here demonstrate that bioactive biomaterials with capacity to bind specific growth factors by design are great targets for regenerative medicine. Supramolecular nanofibers presenting BMP-2-binding epitopes on the surface exhibit superior spinal fusion rates in rats, effectively decreasing the therapeutic dose of BMP-2 by 10-fold. Importantly, the bioactive nanofibers elicit 42{\%} fusion rate without the addition of exogenous BMP-2.",
keywords = "BMP-2 (bone morphogenetic protein-2), Bone regeneration, Peptide amphiphile, Regenerative medicine, Spinal fusion",
author = "Lee, {Sungsoo S.} and Hsu, {Erin L.} and Marco Mendoza and Jason Ghodasra and Nickoli, {Michael S.} and Amruta Ashtekar and Mahesh Polavarapu and Jacob Babu and Riaz, {Rehan M.} and Nicolas, {Joseph D.} and David Nelson and Hashmi, {Sohaib Z.} and Kaltz, {Stuart R.} and Earhart, {Jeffrey S.} and Merk, {Bradley R.} and Mckee, {Jeff S.} and Bairstow, {Shawn F.} and Shah, {Ramille N.} and Hsu, {Wellington K.} and Stupp, {Samuel I}",
year = "2015",
month = "1",
day = "1",
doi = "10.1002/adhm.201400129",
language = "English",
volume = "4",
pages = "131--141",
journal = "Advanced healthcare materials",
issn = "2192-2640",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - Gel Scaffolds of BMP-2-Binding Peptide Amphiphile Nanofibers for Spinal Arthrodesis

AU - Lee, Sungsoo S.

AU - Hsu, Erin L.

AU - Mendoza, Marco

AU - Ghodasra, Jason

AU - Nickoli, Michael S.

AU - Ashtekar, Amruta

AU - Polavarapu, Mahesh

AU - Babu, Jacob

AU - Riaz, Rehan M.

AU - Nicolas, Joseph D.

AU - Nelson, David

AU - Hashmi, Sohaib Z.

AU - Kaltz, Stuart R.

AU - Earhart, Jeffrey S.

AU - Merk, Bradley R.

AU - Mckee, Jeff S.

AU - Bairstow, Shawn F.

AU - Shah, Ramille N.

AU - Hsu, Wellington K.

AU - Stupp, Samuel I

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Peptide amphiphile (PA) nanofibers formed by self-assembly can be customized for specific applications in regenerative medicine through the use of molecules that display bioactive signals on their surfaces. Here, the use of PA nanofibers with binding affinity for the bone promoting growth factor BMP-2 to create a gel scaffold for osteogenesis is reported. With the objective of reducing the amount of BMP-2 used clinically for successful arthrodesis in the spine, amounts of growth factor incorporated in the scaffolds that are 10 to 100 times lower than that those used clinically in collagen scaffolds are used. The efficacy of the bioactive PA system to promote BMP-2-induced osteogenesis in vivo is investigated in a rat posterolateral lumbar intertransverse spinal fusion model. PA nanofiber gels displaying BMP-2-binding segments exhibit superior spinal fusion rates relative to controls, effectively decreasing the required therapeutic dose of BMP-2 by 10-fold. Interestingly, a 42% fusion rate is observed for gels containing the bioactive nanofibers without the use of exogenous BMP-2, suggesting the ability of the nanofiber to recruit endogenous growth factor. Results obtained here demonstrate that bioactive biomaterials with capacity to bind specific growth factors by design are great targets for regenerative medicine. Supramolecular nanofibers presenting BMP-2-binding epitopes on the surface exhibit superior spinal fusion rates in rats, effectively decreasing the therapeutic dose of BMP-2 by 10-fold. Importantly, the bioactive nanofibers elicit 42% fusion rate without the addition of exogenous BMP-2.

AB - Peptide amphiphile (PA) nanofibers formed by self-assembly can be customized for specific applications in regenerative medicine through the use of molecules that display bioactive signals on their surfaces. Here, the use of PA nanofibers with binding affinity for the bone promoting growth factor BMP-2 to create a gel scaffold for osteogenesis is reported. With the objective of reducing the amount of BMP-2 used clinically for successful arthrodesis in the spine, amounts of growth factor incorporated in the scaffolds that are 10 to 100 times lower than that those used clinically in collagen scaffolds are used. The efficacy of the bioactive PA system to promote BMP-2-induced osteogenesis in vivo is investigated in a rat posterolateral lumbar intertransverse spinal fusion model. PA nanofiber gels displaying BMP-2-binding segments exhibit superior spinal fusion rates relative to controls, effectively decreasing the required therapeutic dose of BMP-2 by 10-fold. Interestingly, a 42% fusion rate is observed for gels containing the bioactive nanofibers without the use of exogenous BMP-2, suggesting the ability of the nanofiber to recruit endogenous growth factor. Results obtained here demonstrate that bioactive biomaterials with capacity to bind specific growth factors by design are great targets for regenerative medicine. Supramolecular nanofibers presenting BMP-2-binding epitopes on the surface exhibit superior spinal fusion rates in rats, effectively decreasing the therapeutic dose of BMP-2 by 10-fold. Importantly, the bioactive nanofibers elicit 42% fusion rate without the addition of exogenous BMP-2.

KW - BMP-2 (bone morphogenetic protein-2)

KW - Bone regeneration

KW - Peptide amphiphile

KW - Regenerative medicine

KW - Spinal fusion

UR - http://www.scopus.com/inward/record.url?scp=84920705383&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84920705383&partnerID=8YFLogxK

U2 - 10.1002/adhm.201400129

DO - 10.1002/adhm.201400129

M3 - Article

VL - 4

SP - 131

EP - 141

JO - Advanced healthcare materials

JF - Advanced healthcare materials

SN - 2192-2640

IS - 1

ER -