Genetic analysis of the Photosystem I subunits from the red alga, Galdieria sulphuraria

Christopher Vanselow, Andreas P M Weber, Kirsten Krause, Petra Fromme

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Currently, there are very little data available regarding the photosynthetic apparatus of red algae. We have analyzed the genes for Photosystem I in the recently sequenced genome of the red alga Galdieria sulphuraria. All subunits that are conserved between plants and cyanobacteria were unambiguously identified in the Galdieria genome: PsaA, PsaB, PsaC, PsaD, PsaE, PsaF, PsaI, PsaJ, PsaK and PsaL. From the plant specific subunits, PsaN and PsaO were identified but the sequence homology was much lower than for the subunits that are present in plants and cyanobacteria. The subunit PsaX, which is specific for thermophilic cyanobacteria, is not present in the Galdieria genome, whereas PsaM is a plastid-encoded protein as in other red algae. The sequences of the core subunits of PSI were further analyzed by mapping of the conserved areas in the crystal structures of cyanobacterial and plant PSI. The structural comparison shows that PSI from the red alga Galdieria may represent a common ancestral structure at the interface between cyanobacterial and plant PSI. Some subunits have a "zwitter" structure that contains structural elements that show similarities with either plant or cyanobacterial PSI. The structure of PsaL, which is responsible for the trimerization of PSI in cyanobacteria, lacks a short helix and the Ca2+ binding site, which are essential for trimer formation indicating that the Galdieria PSI is a monomer. However the sequence homology to plant PsaL is low and lacks strong conservation of the interaction sites with PsaH. Furthermore, the sites for interaction of plant PSI with the LHCI complex are not well conserved between plants and Galdieria, which may indicate that Galdieria may contain a PSI that is evolutionarily much more ancient than PSI from green algae, plants and the current cyanobacteria.

Original languageEnglish
Pages (from-to)46-59
Number of pages14
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1787
Issue number1
DOIs
Publication statusPublished - Jan 2009

Fingerprint

Photosystem I Protein Complex
Rhodophyta
Algae
Cyanobacteria
Genes
Genome
Sequence Homology
Viridiplantae
Chloroplast Proteins
Conservation
Plant Structures
Monomers
Crystal structure
Chlorophyta
Binding Sites
Proteins

Keywords

  • Electron transfer
  • Evolution
  • Gene identification
  • Light harvesting
  • Photosynthesis
  • Photosystem I
  • Red algae

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Genetic analysis of the Photosystem I subunits from the red alga, Galdieria sulphuraria. / Vanselow, Christopher; Weber, Andreas P M; Krause, Kirsten; Fromme, Petra.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1787, No. 1, 01.2009, p. 46-59.

Research output: Contribution to journalArticle

Vanselow, Christopher ; Weber, Andreas P M ; Krause, Kirsten ; Fromme, Petra. / Genetic analysis of the Photosystem I subunits from the red alga, Galdieria sulphuraria. In: Biochimica et Biophysica Acta - Bioenergetics. 2009 ; Vol. 1787, No. 1. pp. 46-59.
@article{056dc808fe5f466d871deada95ebf165,
title = "Genetic analysis of the Photosystem I subunits from the red alga, Galdieria sulphuraria",
abstract = "Currently, there are very little data available regarding the photosynthetic apparatus of red algae. We have analyzed the genes for Photosystem I in the recently sequenced genome of the red alga Galdieria sulphuraria. All subunits that are conserved between plants and cyanobacteria were unambiguously identified in the Galdieria genome: PsaA, PsaB, PsaC, PsaD, PsaE, PsaF, PsaI, PsaJ, PsaK and PsaL. From the plant specific subunits, PsaN and PsaO were identified but the sequence homology was much lower than for the subunits that are present in plants and cyanobacteria. The subunit PsaX, which is specific for thermophilic cyanobacteria, is not present in the Galdieria genome, whereas PsaM is a plastid-encoded protein as in other red algae. The sequences of the core subunits of PSI were further analyzed by mapping of the conserved areas in the crystal structures of cyanobacterial and plant PSI. The structural comparison shows that PSI from the red alga Galdieria may represent a common ancestral structure at the interface between cyanobacterial and plant PSI. Some subunits have a {"}zwitter{"} structure that contains structural elements that show similarities with either plant or cyanobacterial PSI. The structure of PsaL, which is responsible for the trimerization of PSI in cyanobacteria, lacks a short helix and the Ca2+ binding site, which are essential for trimer formation indicating that the Galdieria PSI is a monomer. However the sequence homology to plant PsaL is low and lacks strong conservation of the interaction sites with PsaH. Furthermore, the sites for interaction of plant PSI with the LHCI complex are not well conserved between plants and Galdieria, which may indicate that Galdieria may contain a PSI that is evolutionarily much more ancient than PSI from green algae, plants and the current cyanobacteria.",
keywords = "Electron transfer, Evolution, Gene identification, Light harvesting, Photosynthesis, Photosystem I, Red algae",
author = "Christopher Vanselow and Weber, {Andreas P M} and Kirsten Krause and Petra Fromme",
year = "2009",
month = "1",
doi = "10.1016/j.bbabio.2008.10.004",
language = "English",
volume = "1787",
pages = "46--59",
journal = "Biochimica et Biophysica Acta - Bioenergetics",
issn = "0005-2728",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Genetic analysis of the Photosystem I subunits from the red alga, Galdieria sulphuraria

AU - Vanselow, Christopher

AU - Weber, Andreas P M

AU - Krause, Kirsten

AU - Fromme, Petra

PY - 2009/1

Y1 - 2009/1

N2 - Currently, there are very little data available regarding the photosynthetic apparatus of red algae. We have analyzed the genes for Photosystem I in the recently sequenced genome of the red alga Galdieria sulphuraria. All subunits that are conserved between plants and cyanobacteria were unambiguously identified in the Galdieria genome: PsaA, PsaB, PsaC, PsaD, PsaE, PsaF, PsaI, PsaJ, PsaK and PsaL. From the plant specific subunits, PsaN and PsaO were identified but the sequence homology was much lower than for the subunits that are present in plants and cyanobacteria. The subunit PsaX, which is specific for thermophilic cyanobacteria, is not present in the Galdieria genome, whereas PsaM is a plastid-encoded protein as in other red algae. The sequences of the core subunits of PSI were further analyzed by mapping of the conserved areas in the crystal structures of cyanobacterial and plant PSI. The structural comparison shows that PSI from the red alga Galdieria may represent a common ancestral structure at the interface between cyanobacterial and plant PSI. Some subunits have a "zwitter" structure that contains structural elements that show similarities with either plant or cyanobacterial PSI. The structure of PsaL, which is responsible for the trimerization of PSI in cyanobacteria, lacks a short helix and the Ca2+ binding site, which are essential for trimer formation indicating that the Galdieria PSI is a monomer. However the sequence homology to plant PsaL is low and lacks strong conservation of the interaction sites with PsaH. Furthermore, the sites for interaction of plant PSI with the LHCI complex are not well conserved between plants and Galdieria, which may indicate that Galdieria may contain a PSI that is evolutionarily much more ancient than PSI from green algae, plants and the current cyanobacteria.

AB - Currently, there are very little data available regarding the photosynthetic apparatus of red algae. We have analyzed the genes for Photosystem I in the recently sequenced genome of the red alga Galdieria sulphuraria. All subunits that are conserved between plants and cyanobacteria were unambiguously identified in the Galdieria genome: PsaA, PsaB, PsaC, PsaD, PsaE, PsaF, PsaI, PsaJ, PsaK and PsaL. From the plant specific subunits, PsaN and PsaO were identified but the sequence homology was much lower than for the subunits that are present in plants and cyanobacteria. The subunit PsaX, which is specific for thermophilic cyanobacteria, is not present in the Galdieria genome, whereas PsaM is a plastid-encoded protein as in other red algae. The sequences of the core subunits of PSI were further analyzed by mapping of the conserved areas in the crystal structures of cyanobacterial and plant PSI. The structural comparison shows that PSI from the red alga Galdieria may represent a common ancestral structure at the interface between cyanobacterial and plant PSI. Some subunits have a "zwitter" structure that contains structural elements that show similarities with either plant or cyanobacterial PSI. The structure of PsaL, which is responsible for the trimerization of PSI in cyanobacteria, lacks a short helix and the Ca2+ binding site, which are essential for trimer formation indicating that the Galdieria PSI is a monomer. However the sequence homology to plant PsaL is low and lacks strong conservation of the interaction sites with PsaH. Furthermore, the sites for interaction of plant PSI with the LHCI complex are not well conserved between plants and Galdieria, which may indicate that Galdieria may contain a PSI that is evolutionarily much more ancient than PSI from green algae, plants and the current cyanobacteria.

KW - Electron transfer

KW - Evolution

KW - Gene identification

KW - Light harvesting

KW - Photosynthesis

KW - Photosystem I

KW - Red algae

UR - http://www.scopus.com/inward/record.url?scp=57649143641&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57649143641&partnerID=8YFLogxK

U2 - 10.1016/j.bbabio.2008.10.004

DO - 10.1016/j.bbabio.2008.10.004

M3 - Article

VL - 1787

SP - 46

EP - 59

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

SN - 0005-2728

IS - 1

ER -