'Giant' multishell CdSe nanocrystal quantum dots with suppressed blinking

Novel fluorescent probes for real-time detection of single-molecule events

Jennifer A. Hollingsworth, Javier Vela, Yongfen Chen, Han Htoon, Victor I Klimov, Amy R. Casson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

We reported for the first time that key nanocrystal quantum dot (NQD) optical properties-quantum yield, photobleaching and blinking-can be rendered independent of NQD surface chemistry and environment by growth of a very thick, defect-free inorganic shell (Chen, et al. J. Am. Chem. Soc. 2008). Here, we show the precise shell-thickness dependence of these effects. We demonstrate that 'giant-shell' NQDs can be largely non-blinking for observation times as long as 54 minutes and that on-time fractions are independent of experimental time-resolution from 1-200 ms. These effects are primarily demonstrated on (CdSe)CdS (core)shell NQDs, but we also show that alloyed shells comprising CdxZn1-xS and terminated with a non-cytotoxic ZnS layer exhibit similar properties. The mechanism for suppressed blinking and dramatically enhanced stability is attributed to both effective isolation of the NQD core excitonic wavefunction from the NQD surface, as well as a quasi-Type II electronic structure. The unusual electronic structure provides for effective spatial separation of the electron and hole into the shell and core, respectively, and, thereby, for reduced efficiencies in non-radiative Auger recombination.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7189
DOIs
Publication statusPublished - 2009
EventColloidal Quantum Dots for Biomedical Applications IV - San Jose, CA, United States
Duration: Jan 24 2009Jan 26 2009

Other

OtherColloidal Quantum Dots for Biomedical Applications IV
CountryUnited States
CitySan Jose, CA
Period1/24/091/26/09

Fingerprint

blinking
Blinking
Quantum Dots
Fluorescent Dyes
Nanoparticles
Nanocrystals
Semiconductor quantum dots
nanocrystals
quantum dots
Molecules
probes
Electronic structure
molecules
electronic structure
Photobleaching
Quantum yield
Wave functions
Surface chemistry
Genetic Recombination
isolation

Keywords

  • Core-shell
  • Nanocrystal quantum dot
  • Non-blinking
  • Suppressed blinking

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Hollingsworth, J. A., Vela, J., Chen, Y., Htoon, H., Klimov, V. I., & Casson, A. R. (2009). 'Giant' multishell CdSe nanocrystal quantum dots with suppressed blinking: Novel fluorescent probes for real-time detection of single-molecule events. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7189). [718904] https://doi.org/10.1117/12.809678

'Giant' multishell CdSe nanocrystal quantum dots with suppressed blinking : Novel fluorescent probes for real-time detection of single-molecule events. / Hollingsworth, Jennifer A.; Vela, Javier; Chen, Yongfen; Htoon, Han; Klimov, Victor I; Casson, Amy R.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7189 2009. 718904.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hollingsworth, JA, Vela, J, Chen, Y, Htoon, H, Klimov, VI & Casson, AR 2009, 'Giant' multishell CdSe nanocrystal quantum dots with suppressed blinking: Novel fluorescent probes for real-time detection of single-molecule events. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7189, 718904, Colloidal Quantum Dots for Biomedical Applications IV, San Jose, CA, United States, 1/24/09. https://doi.org/10.1117/12.809678
Hollingsworth JA, Vela J, Chen Y, Htoon H, Klimov VI, Casson AR. 'Giant' multishell CdSe nanocrystal quantum dots with suppressed blinking: Novel fluorescent probes for real-time detection of single-molecule events. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7189. 2009. 718904 https://doi.org/10.1117/12.809678
Hollingsworth, Jennifer A. ; Vela, Javier ; Chen, Yongfen ; Htoon, Han ; Klimov, Victor I ; Casson, Amy R. / 'Giant' multishell CdSe nanocrystal quantum dots with suppressed blinking : Novel fluorescent probes for real-time detection of single-molecule events. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7189 2009.
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