Gold-based SERS tags for biomedical imaging

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The use of SERS tags in biomedical imaging is described. SERS tags are a novel entity that has recently emerged in the SERS community, mainly spurred by the necessity of carrying out experiments in the biomedical and clinical fields, where the heterogeneity and constant evolution of the environment hamper the application of direct SERS sensing concepts. Direct sensing would in fact require the use of nanoparticles with bare metallic surfaces to allow for intense signal responses; however, the high salt contents typical of physiological conditions and issues such as fouling lead the nanoparticles to aggregate and precipitate out of solution, thus limiting reproducibility and quantitative target identification. As a consequence, the concept of indirect detection has gained importance, in which the SERS signal provided by the SERS tag indirectly provides identification and localization of the target. In this brief review, aimed both at the expert scientist and the novice, the anatomy of a SERS tag is first described, which includes the gold nanoparticle, Raman reporter molecules, a coating layer, and targeting moieties, and the concept of hot spot is explained. A brief overview of the most recent imaging applications in vitro, ex vivo, and in vivo is also provided, along with specific recommendations toward the synthesis of effective SERS tags that could find application in the biomedical field, and meet specific needs of the clinical community. Major emphasis is placed on the concept of multiplexing, which is perhaps the most important feature of SERS tags that could render their clinical application a reality.

Original languageEnglish
Article number114002
JournalJournal of Optics (United Kingdom)
Volume17
Issue number11
DOIs
Publication statusPublished - Oct 23 2015

Fingerprint

Gold
gold
Imaging techniques
Nanoparticles
nanoparticles
fouling
anatomy
Fouling
multiplexing
Multiplexing
recommendations
Precipitates
precipitates
Salts
salts
coatings
Coatings
Molecules
synthesis
molecules

Keywords

  • biomedical applications
  • gold nanoparticles
  • imaging
  • SERS
  • tag

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials

Cite this

Gold-based SERS tags for biomedical imaging. / Fabris, Laura.

In: Journal of Optics (United Kingdom), Vol. 17, No. 11, 114002, 23.10.2015.

Research output: Contribution to journalArticle

@article{5a2b34339c7648d48f648f7b68475b51,
title = "Gold-based SERS tags for biomedical imaging",
abstract = "The use of SERS tags in biomedical imaging is described. SERS tags are a novel entity that has recently emerged in the SERS community, mainly spurred by the necessity of carrying out experiments in the biomedical and clinical fields, where the heterogeneity and constant evolution of the environment hamper the application of direct SERS sensing concepts. Direct sensing would in fact require the use of nanoparticles with bare metallic surfaces to allow for intense signal responses; however, the high salt contents typical of physiological conditions and issues such as fouling lead the nanoparticles to aggregate and precipitate out of solution, thus limiting reproducibility and quantitative target identification. As a consequence, the concept of indirect detection has gained importance, in which the SERS signal provided by the SERS tag indirectly provides identification and localization of the target. In this brief review, aimed both at the expert scientist and the novice, the anatomy of a SERS tag is first described, which includes the gold nanoparticle, Raman reporter molecules, a coating layer, and targeting moieties, and the concept of hot spot is explained. A brief overview of the most recent imaging applications in vitro, ex vivo, and in vivo is also provided, along with specific recommendations toward the synthesis of effective SERS tags that could find application in the biomedical field, and meet specific needs of the clinical community. Major emphasis is placed on the concept of multiplexing, which is perhaps the most important feature of SERS tags that could render their clinical application a reality.",
keywords = "biomedical applications, gold nanoparticles, imaging, SERS, tag",
author = "Laura Fabris",
year = "2015",
month = "10",
day = "23",
doi = "10.1088/2040-8978/17/11/114002",
language = "English",
volume = "17",
journal = "Journal of Optics",
issn = "2040-8978",
publisher = "IOP Publishing Ltd.",
number = "11",

}

TY - JOUR

T1 - Gold-based SERS tags for biomedical imaging

AU - Fabris, Laura

PY - 2015/10/23

Y1 - 2015/10/23

N2 - The use of SERS tags in biomedical imaging is described. SERS tags are a novel entity that has recently emerged in the SERS community, mainly spurred by the necessity of carrying out experiments in the biomedical and clinical fields, where the heterogeneity and constant evolution of the environment hamper the application of direct SERS sensing concepts. Direct sensing would in fact require the use of nanoparticles with bare metallic surfaces to allow for intense signal responses; however, the high salt contents typical of physiological conditions and issues such as fouling lead the nanoparticles to aggregate and precipitate out of solution, thus limiting reproducibility and quantitative target identification. As a consequence, the concept of indirect detection has gained importance, in which the SERS signal provided by the SERS tag indirectly provides identification and localization of the target. In this brief review, aimed both at the expert scientist and the novice, the anatomy of a SERS tag is first described, which includes the gold nanoparticle, Raman reporter molecules, a coating layer, and targeting moieties, and the concept of hot spot is explained. A brief overview of the most recent imaging applications in vitro, ex vivo, and in vivo is also provided, along with specific recommendations toward the synthesis of effective SERS tags that could find application in the biomedical field, and meet specific needs of the clinical community. Major emphasis is placed on the concept of multiplexing, which is perhaps the most important feature of SERS tags that could render their clinical application a reality.

AB - The use of SERS tags in biomedical imaging is described. SERS tags are a novel entity that has recently emerged in the SERS community, mainly spurred by the necessity of carrying out experiments in the biomedical and clinical fields, where the heterogeneity and constant evolution of the environment hamper the application of direct SERS sensing concepts. Direct sensing would in fact require the use of nanoparticles with bare metallic surfaces to allow for intense signal responses; however, the high salt contents typical of physiological conditions and issues such as fouling lead the nanoparticles to aggregate and precipitate out of solution, thus limiting reproducibility and quantitative target identification. As a consequence, the concept of indirect detection has gained importance, in which the SERS signal provided by the SERS tag indirectly provides identification and localization of the target. In this brief review, aimed both at the expert scientist and the novice, the anatomy of a SERS tag is first described, which includes the gold nanoparticle, Raman reporter molecules, a coating layer, and targeting moieties, and the concept of hot spot is explained. A brief overview of the most recent imaging applications in vitro, ex vivo, and in vivo is also provided, along with specific recommendations toward the synthesis of effective SERS tags that could find application in the biomedical field, and meet specific needs of the clinical community. Major emphasis is placed on the concept of multiplexing, which is perhaps the most important feature of SERS tags that could render their clinical application a reality.

KW - biomedical applications

KW - gold nanoparticles

KW - imaging

KW - SERS

KW - tag

UR - http://www.scopus.com/inward/record.url?scp=84946762985&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84946762985&partnerID=8YFLogxK

U2 - 10.1088/2040-8978/17/11/114002

DO - 10.1088/2040-8978/17/11/114002

M3 - Article

AN - SCOPUS:84946762985

VL - 17

JO - Journal of Optics

JF - Journal of Optics

SN - 2040-8978

IS - 11

M1 - 114002

ER -