Abstract
The delivery of bioactive molecules into cells has broad applications in biology and medicine. Polymer-modified graphene oxide (GO) has recently emerged as a de facto noncovalent vehicle for hydrophobic drugs. Here, we investigate a different approach using native GO to deliver hydrophilic molecules by co-incubation in culture. GO adsorption and delivery were systematically studied with a library of 15 molecules synthesized with Gd(III) labels to enable quantitation. Amines were revealed to be a key chemical group for adsorption, while delivery was shown to be quantitatively predictable by molecular adsorption, GO sedimentation, and GO size. GO co-incubation was shown to enhance delivery by up to 13-fold and allowed for a 100-fold increase in molecular incubation concentration compared to the alternative of nanoconjugation. When tested in the application of Gd(III) cellular MRI, these advantages led to a nearly 10-fold improvement in sensitivity over the state-of-the-art. GO co-incubation is an effective method of cellular delivery that is easily adoptable by researchers across all fields.
| Original language | English |
|---|---|
| Pages (from-to) | 10168-10177 |
| Number of pages | 10 |
| Journal | ACS Nano |
| Volume | 8 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 28 2014 |
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Keywords
- adsorption
- cell culture
- delivery vehicle
- gadolinium
- graphene oxide
- sedimentation
- surface interaction
ASJC Scopus subject areas
- Engineering(all)
- Materials Science(all)
- Physics and Astronomy(all)
Cite this
Graphene oxide enhances cellular delivery of hydrophilic small molecules by co-incubation. / Hung, Andy H.; Holbrook, Robert J.; Rotz, Matthew W.; Glasscock, Cameron J.; Mansukhani, Nikhita D.; Macrenaris, Keith W.; Manus, Lisa M.; Duch, Matthew C.; Dam, Kevin T.; Hersam, Mark C; Meade, Thomas J.
In: ACS Nano, Vol. 8, No. 10, 28.10.2014, p. 10168-10177.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Graphene oxide enhances cellular delivery of hydrophilic small molecules by co-incubation
AU - Hung, Andy H.
AU - Holbrook, Robert J.
AU - Rotz, Matthew W.
AU - Glasscock, Cameron J.
AU - Mansukhani, Nikhita D.
AU - Macrenaris, Keith W.
AU - Manus, Lisa M.
AU - Duch, Matthew C.
AU - Dam, Kevin T.
AU - Hersam, Mark C
AU - Meade, Thomas J.
PY - 2014/10/28
Y1 - 2014/10/28
N2 - The delivery of bioactive molecules into cells has broad applications in biology and medicine. Polymer-modified graphene oxide (GO) has recently emerged as a de facto noncovalent vehicle for hydrophobic drugs. Here, we investigate a different approach using native GO to deliver hydrophilic molecules by co-incubation in culture. GO adsorption and delivery were systematically studied with a library of 15 molecules synthesized with Gd(III) labels to enable quantitation. Amines were revealed to be a key chemical group for adsorption, while delivery was shown to be quantitatively predictable by molecular adsorption, GO sedimentation, and GO size. GO co-incubation was shown to enhance delivery by up to 13-fold and allowed for a 100-fold increase in molecular incubation concentration compared to the alternative of nanoconjugation. When tested in the application of Gd(III) cellular MRI, these advantages led to a nearly 10-fold improvement in sensitivity over the state-of-the-art. GO co-incubation is an effective method of cellular delivery that is easily adoptable by researchers across all fields.
AB - The delivery of bioactive molecules into cells has broad applications in biology and medicine. Polymer-modified graphene oxide (GO) has recently emerged as a de facto noncovalent vehicle for hydrophobic drugs. Here, we investigate a different approach using native GO to deliver hydrophilic molecules by co-incubation in culture. GO adsorption and delivery were systematically studied with a library of 15 molecules synthesized with Gd(III) labels to enable quantitation. Amines were revealed to be a key chemical group for adsorption, while delivery was shown to be quantitatively predictable by molecular adsorption, GO sedimentation, and GO size. GO co-incubation was shown to enhance delivery by up to 13-fold and allowed for a 100-fold increase in molecular incubation concentration compared to the alternative of nanoconjugation. When tested in the application of Gd(III) cellular MRI, these advantages led to a nearly 10-fold improvement in sensitivity over the state-of-the-art. GO co-incubation is an effective method of cellular delivery that is easily adoptable by researchers across all fields.
KW - adsorption
KW - cell culture
KW - delivery vehicle
KW - gadolinium
KW - graphene oxide
KW - sedimentation
KW - surface interaction
UR - http://www.scopus.com/inward/record.url?scp=84908426153&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84908426153&partnerID=8YFLogxK
U2 - 10.1021/nn502986e
DO - 10.1021/nn502986e
M3 - Article
C2 - 25226566
AN - SCOPUS:84908426153
VL - 8
SP - 10168
EP - 10177
JO - ACS Nano
JF - ACS Nano
SN - 1936-0851
IS - 10
ER -