Growth factor delivery from self-assembling nanofibers to facilitate islet transplantation

John C. Stendahl, Ling Jia Wang, Lesley W. Chow, Dixon B. Kaufman, Samuel I. Stupp

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Recent advances in nanotechnology and molecular self-assembly may provide novel solutions to current cell transplantation deficiencies. Heparin-binding peptide amphiphiles (HBPAs) self-assemble from aqueous media into nanofibers that bind growth factors through interactions with the bioactive polymer heparin. In this report, we demonstrate that delivery of vascular endothelial growth factor and fibroblast growth factor-2 from HBPA scaffolds significantly increases blood vessel density in the mouse omentum over control scaffolds without growth factors (P<0.0005) and significantly enhances islet engraftment. Diabetic recipients transplanted with 250 isologous islets and HBPA scaffolds containing vascular endothelial growth factor/fibroblast growth factor-2 achieved normoglycemia at a higher rate (78%) than control animals receiving identical scaffolds without growth factors (30%; P<0.05) or growth factors alone (20%). These data indicate that the enhanced engraftment can be attributed to specific growth factor effects that were made possible by the delivery mechanism of HBPA nanostructures.

Original languageEnglish
Pages (from-to)478-481
Number of pages4
JournalTransplantation
Volume86
Issue number3
DOIs
Publication statusPublished - Aug 15 2008

Keywords

  • Fibroblast growth factor-2
  • Islet transplantation
  • Omentum
  • Peptide amphiphile nanofibers
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Transplantation

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