Hierarchical assembly of plasmonic nanostructures using virus capsid scaffolds on DNA origami templates

Debin Wang, Stacy L. Capehart, Suchetan Pal, Minghui Liu, Lei Zhang, P. James Schuck, Yan Liu, Hao Yan, Matthew B. Francis, James J. De Yoreo

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Building plasmonic nanostructures using biomolecules as scaffolds has shown great potential for attaining tunable light absorption and emission via precise spatial organization of optical species and antennae. Here we report bottom-up assembly of hierarchical plasmonic nanostructures using DNA origami templates and MS2 virus capsids. These serve as programmable scaffolds that provide molecular level control over the distribution of fluorophores and nanometer-scale control over their distance from a gold nanoparticle antenna. While previous research using DNA origami to assemble plasmonic nanostructures focused on determining the distance-dependent response of single fluorophores, here we address the challenge of constructing hybrid nanostructures that present an organized ensemble of fluorophores and then investigate the plasmonic response. By combining finite-difference time-domain numerical simulations with atomic force microscopy and correlated scanning confocal fluorescence microscopy, we find that the use of the scaffold keeps the majority of the fluorophores out of the quenching zone, leading to increased fluorescence intensity and mild levels of enhancement. The results show that the degree of enhancement can be controlled by exploiting capsid scaffolds of different sizes and tuning capsid-AuNP distances. These bioinspired plasmonic nanostructures provide a flexible design for manipulating photonic excitation and photoemission.

Original languageEnglish
Pages (from-to)7896-7904
Number of pages9
JournalACS Nano
Volume8
Issue number8
DOIs
Publication statusPublished - 2014

Fingerprint

Nanostructures
Capsid
viruses
Viruses
Scaffolds
Fluorophores
DNA
templates
deoxyribonucleic acid
assembly
antennas
fluorescence
augmentation
electromagnetic absorption
light emission
Optics and Photonics
Antennas
photoelectric emission
quenching
tuning

Keywords

  • atomic force microscopy (AFM)
  • bioinspired assembly
  • DNA origami
  • finite-difference time-domain (FDTD) simulation
  • plasmonic nanostructure
  • scanning confocal microscopy
  • virus capsid

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)
  • Medicine(all)

Cite this

Wang, D., Capehart, S. L., Pal, S., Liu, M., Zhang, L., Schuck, P. J., ... De Yoreo, J. J. (2014). Hierarchical assembly of plasmonic nanostructures using virus capsid scaffolds on DNA origami templates. ACS Nano, 8(8), 7896-7904. https://doi.org/10.1021/nn5015819

Hierarchical assembly of plasmonic nanostructures using virus capsid scaffolds on DNA origami templates. / Wang, Debin; Capehart, Stacy L.; Pal, Suchetan; Liu, Minghui; Zhang, Lei; Schuck, P. James; Liu, Yan; Yan, Hao; Francis, Matthew B.; De Yoreo, James J.

In: ACS Nano, Vol. 8, No. 8, 2014, p. 7896-7904.

Research output: Contribution to journalArticle

Wang, D, Capehart, SL, Pal, S, Liu, M, Zhang, L, Schuck, PJ, Liu, Y, Yan, H, Francis, MB & De Yoreo, JJ 2014, 'Hierarchical assembly of plasmonic nanostructures using virus capsid scaffolds on DNA origami templates', ACS Nano, vol. 8, no. 8, pp. 7896-7904. https://doi.org/10.1021/nn5015819
Wang, Debin ; Capehart, Stacy L. ; Pal, Suchetan ; Liu, Minghui ; Zhang, Lei ; Schuck, P. James ; Liu, Yan ; Yan, Hao ; Francis, Matthew B. ; De Yoreo, James J. / Hierarchical assembly of plasmonic nanostructures using virus capsid scaffolds on DNA origami templates. In: ACS Nano. 2014 ; Vol. 8, No. 8. pp. 7896-7904.
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