High resolution crystal structure of ferricytochrome c' from Rhodobacter sphaeroides

Laura M.-Ramirez, Herbert L. Axelrod, Steven R. Herron, Bernhard Rupp, James Paul Allen, Katherine A. Kantardjieff

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Cytochrome c' isolated from Rhodobacter sphaeroides strain R26 (RSCP) crystallizes as a dimer of two identical 14-kDa subunits, in trigonal space group P31, with cell parameters a, b = 48.10 Å, c = 115.80 Å. The crystal structure of RSCP has been solved by molecular replacement using cytochrome c' from Rhodobacter capsulatus (PDB ID: 1CPQ) as a search model. To ensure effective phase bias removal, the RSCP model was iteratively built into maps generated by a modified wARP procedure, Shake&wARP. The 1.8 Å model (PDB ID: 1GQA) has been refined to an R = 0.204 and freeR = 0.254. Each subunit consists of four antiparallel α-helices, with the pentacoordinate heme covalently bound to a C-X-Y-C-H motif near the C-terminus. F14, located on helix A, blocks direct access to what would be the sixth "distal" ligand binding site of the heme. The dimer subunits form a flattened "X" shape, intermediate between the Type 1 and Type 2 cytochromes c'. The presence of the aromatic F14 and a deep channel between helices B and C places RSCP into Group 1 cytochromes c'. Clear electron density has revealed that the amino acid sequences for the cytochrome c' from strains R26 and 2.4.1 are identical.

Original languageEnglish
Pages (from-to)413-424
Number of pages12
JournalJournal of Chemical Crystallography
Volume33
Issue number5-6
DOIs
Publication statusPublished - Jun 2003

Fingerprint

Cytochromes c'
Rhodobacter sphaeroides
cytochromes
Crystal structure
crystal structure
helices
high resolution
Heme
Dimers
dimers
Rhodobacter capsulatus
Carrier concentration
amino acids
Amino Acid Sequence
Binding Sites
Electrons
Ligands
Amino Acids
ligands
cells

Keywords

  • Cytochrome c'
  • Four helix bundle
  • Heme protein
  • Rhodobacter sphaeroides

ASJC Scopus subject areas

  • Spectroscopy
  • Condensed Matter Physics
  • Structural Biology

Cite this

High resolution crystal structure of ferricytochrome c' from Rhodobacter sphaeroides. / M.-Ramirez, Laura; Axelrod, Herbert L.; Herron, Steven R.; Rupp, Bernhard; Allen, James Paul; Kantardjieff, Katherine A.

In: Journal of Chemical Crystallography, Vol. 33, No. 5-6, 06.2003, p. 413-424.

Research output: Contribution to journalArticle

M.-Ramirez, Laura ; Axelrod, Herbert L. ; Herron, Steven R. ; Rupp, Bernhard ; Allen, James Paul ; Kantardjieff, Katherine A. / High resolution crystal structure of ferricytochrome c' from Rhodobacter sphaeroides. In: Journal of Chemical Crystallography. 2003 ; Vol. 33, No. 5-6. pp. 413-424.
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