High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography

Sébastien Boutet, Lukas Lomb, Garth J. Williams, Thomas R M Barends, Andrew Aquila, R. Bruce Doak, Uwe Weierstall, Daniel P. DePonte, Jan Steinbrener, Robert L. Shoeman, Marc Messerschmidt, Anton Barty, Thomas A. White, Stephan Kassemeyer, Richard A. Kirian, M. Marvin Seibert, Paul A. Montanez, Chris Kenney, Ryan Herbst, Philip Hart & 34 others Jack Pines, Gunther Haller, Sol M. Gruner, Hugh T. Philipp, Mark W. Tate, Marianne Hromalik, Lucas J. Koerner, Niels Van Bakel, John Morse, Wilfred Ghonsalves, David Arnlund, Michael J. Bogan, Carl Caleman, Raimund Fromme, Christina Y. Hampton, Mark S. Hunter, Linda C. Johansson, Gergely Katona, Christopher Kupitz, Mengning Liang, Andrew V. Martin, Karol Nass, Lars Redecke, Francesco Stellato, Nicusor Timneanu, Dingjie Wang, Nadia A. Zatsepin, Donald Schafer, James Defever, Richard Neutze, Petra Fromme, John C H Spence, Henry N. Chapman, Ilme Schlichting

Research output: Contribution to journalArticle

476 Citations (Scopus)

Abstract

Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.

Original languageEnglish
Pages (from-to)362-364
Number of pages3
JournalScience
Volume337
Issue number6092
DOIs
Publication statusPublished - Jul 20 2012

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Crystallography
X-Rays
Synchrotrons
Muramidase
Proteins
Lasers
Electrons
Radiation
Growth

ASJC Scopus subject areas

  • General

Cite this

Boutet, S., Lomb, L., Williams, G. J., Barends, T. R. M., Aquila, A., Doak, R. B., ... Schlichting, I. (2012). High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography. Science, 337(6092), 362-364. https://doi.org/10.1126/science.1217737

High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography. / Boutet, Sébastien; Lomb, Lukas; Williams, Garth J.; Barends, Thomas R M; Aquila, Andrew; Doak, R. Bruce; Weierstall, Uwe; DePonte, Daniel P.; Steinbrener, Jan; Shoeman, Robert L.; Messerschmidt, Marc; Barty, Anton; White, Thomas A.; Kassemeyer, Stephan; Kirian, Richard A.; Seibert, M. Marvin; Montanez, Paul A.; Kenney, Chris; Herbst, Ryan; Hart, Philip; Pines, Jack; Haller, Gunther; Gruner, Sol M.; Philipp, Hugh T.; Tate, Mark W.; Hromalik, Marianne; Koerner, Lucas J.; Van Bakel, Niels; Morse, John; Ghonsalves, Wilfred; Arnlund, David; Bogan, Michael J.; Caleman, Carl; Fromme, Raimund; Hampton, Christina Y.; Hunter, Mark S.; Johansson, Linda C.; Katona, Gergely; Kupitz, Christopher; Liang, Mengning; Martin, Andrew V.; Nass, Karol; Redecke, Lars; Stellato, Francesco; Timneanu, Nicusor; Wang, Dingjie; Zatsepin, Nadia A.; Schafer, Donald; Defever, James; Neutze, Richard; Fromme, Petra; Spence, John C H; Chapman, Henry N.; Schlichting, Ilme.

In: Science, Vol. 337, No. 6092, 20.07.2012, p. 362-364.

Research output: Contribution to journalArticle

Boutet, S, Lomb, L, Williams, GJ, Barends, TRM, Aquila, A, Doak, RB, Weierstall, U, DePonte, DP, Steinbrener, J, Shoeman, RL, Messerschmidt, M, Barty, A, White, TA, Kassemeyer, S, Kirian, RA, Seibert, MM, Montanez, PA, Kenney, C, Herbst, R, Hart, P, Pines, J, Haller, G, Gruner, SM, Philipp, HT, Tate, MW, Hromalik, M, Koerner, LJ, Van Bakel, N, Morse, J, Ghonsalves, W, Arnlund, D, Bogan, MJ, Caleman, C, Fromme, R, Hampton, CY, Hunter, MS, Johansson, LC, Katona, G, Kupitz, C, Liang, M, Martin, AV, Nass, K, Redecke, L, Stellato, F, Timneanu, N, Wang, D, Zatsepin, NA, Schafer, D, Defever, J, Neutze, R, Fromme, P, Spence, JCH, Chapman, HN & Schlichting, I 2012, 'High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography', Science, vol. 337, no. 6092, pp. 362-364. https://doi.org/10.1126/science.1217737
Boutet S, Lomb L, Williams GJ, Barends TRM, Aquila A, Doak RB et al. High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography. Science. 2012 Jul 20;337(6092):362-364. https://doi.org/10.1126/science.1217737
Boutet, Sébastien ; Lomb, Lukas ; Williams, Garth J. ; Barends, Thomas R M ; Aquila, Andrew ; Doak, R. Bruce ; Weierstall, Uwe ; DePonte, Daniel P. ; Steinbrener, Jan ; Shoeman, Robert L. ; Messerschmidt, Marc ; Barty, Anton ; White, Thomas A. ; Kassemeyer, Stephan ; Kirian, Richard A. ; Seibert, M. Marvin ; Montanez, Paul A. ; Kenney, Chris ; Herbst, Ryan ; Hart, Philip ; Pines, Jack ; Haller, Gunther ; Gruner, Sol M. ; Philipp, Hugh T. ; Tate, Mark W. ; Hromalik, Marianne ; Koerner, Lucas J. ; Van Bakel, Niels ; Morse, John ; Ghonsalves, Wilfred ; Arnlund, David ; Bogan, Michael J. ; Caleman, Carl ; Fromme, Raimund ; Hampton, Christina Y. ; Hunter, Mark S. ; Johansson, Linda C. ; Katona, Gergely ; Kupitz, Christopher ; Liang, Mengning ; Martin, Andrew V. ; Nass, Karol ; Redecke, Lars ; Stellato, Francesco ; Timneanu, Nicusor ; Wang, Dingjie ; Zatsepin, Nadia A. ; Schafer, Donald ; Defever, James ; Neutze, Richard ; Fromme, Petra ; Spence, John C H ; Chapman, Henry N. ; Schlichting, Ilme. / High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography. In: Science. 2012 ; Vol. 337, No. 6092. pp. 362-364.
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AU - Doak, R. Bruce

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AU - Ghonsalves, Wilfred

AU - Arnlund, David

AU - Bogan, Michael J.

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AU - Fromme, Raimund

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