High-speed low-frequency chirped coherent anti-Stokes Raman scattering microscopy using an ultra-steep long-pass filter

Liqing Ren, Dekel Raanan, Ilan Hurwitz, Dan Oron

Research output: Contribution to journalArticle

Abstract

Coherent anti-Stokes Raman scattering (CARS) microscopy is becoming a more common tool in biomedical research. High-speed CARS microscopy has important applications in live cell imaging and in label-free pathology. However, only a few realizations exist of CARS imaging applied in the few terahertz spectral range (<300 cm−1), in which much is unknown to date. Although single-beam CARS microscopy proved to be robust in this low-frequency region, pixel-dwell time using presently available schemes is still relatively long, in the millisecond scale. Single-beam notch-shaped chirped-CARS (C-CARS) microscopy in the fingerprint region can be performed without using lock-in detection, yet it necessitates double-notch shaping, resulting in a relatively complex system. Here, we demonstrate that C-CARS in the low-frequency regime can be achieved using a sharp-edge, which is created by an ultra-steep long-pass filter (ULPF). Furthermore, we demonstrate that this variant of C-CARS spectroscopy can be performed without post-processing analyses. This is used to image collagen in a biological sample with a pixel dwell time of 200 microseconds. This sharp-edge C-CARS method may find important application in rapid low-frequency CARS imaging of live cells or for imaging of fast flowing objects such as in microfluidic channels.

Original languageEnglish
Pages (from-to)35993-36001
Number of pages9
JournalOptics Express
Volume27
Issue number24
DOIs
Publication statusPublished - Jan 1 2019

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high speed
Raman spectra
low frequencies
microscopy
filters
dwell
notches
pixels
pathology
collagens
cells
complex systems
spectroscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

High-speed low-frequency chirped coherent anti-Stokes Raman scattering microscopy using an ultra-steep long-pass filter. / Ren, Liqing; Raanan, Dekel; Hurwitz, Ilan; Oron, Dan.

In: Optics Express, Vol. 27, No. 24, 01.01.2019, p. 35993-36001.

Research output: Contribution to journalArticle

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