High throughput protein nanocrystal fractionation in a microfluidic sorter

Bahige G. Abdallah, Shatabdi Roy-Chowdhury, Jesse Coe, Petra Fromme, Alexandra Ros

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Protein crystallography is transitioning into a new generation with the introduction of the X-ray free electron laser, which can be used to solve the structures of complex proteins via serial femtosecond crystallography. Sample characteristics play a critical role in successful implementation of this new technology, whereby a small, narrow protein crystal size distribution is desired to provide high quality diffraction data. To provide such a sample, we developed a microfluidic device that facilitates dielectrophoretic sorting of heterogeneous particle mixtures into various size fractions. The first generation device demonstrated great potential and success toward this endeavor; thus, in this work, we present a comprehensive optimization study to improve throughput and control over sorting outcomes. First, device geometry was designed considering a variety of criteria, and applied potentials were modeled to determine the scheme achieving the largest sorting efficiency for isolating nanoparticles from microparticles. Further, to investigate sorting efficiency within the nanoparticle regime, critical geometrical dimensions and input parameters were optimized to achieve high sorting efficiencies. Experiments revealed fractionation of nanobeads from microbeads in the optimized device with high sorting efficiencies, and protein crystals were sorted into submicrometer size fractions as desired for future serial femtosecond crystallography experiments.

Original languageEnglish
Pages (from-to)4159-4167
Number of pages9
JournalAnalytical Chemistry
Volume87
Issue number8
DOIs
Publication statusPublished - Apr 21 2015

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ASJC Scopus subject areas

  • Analytical Chemistry

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