High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source

Jose M. Martin-Garcia, Lan Zhu, Derek Mendez, Ming Yue Lee, Eugene Chun, Chufeng Li, Hao Hu, Ganesh Subramanian, David Kissick, Craig Ogata, Robert Henning, Andrii Ishchenko, Zachary Dobson, Shangji Zhang, Uwe Weierstall, John C.H. Spence, Petra Fromme, Nadia A. Zatsepin, Robert F. Fischetti, Vadim CherezovWei Liu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Since the first successful serial crystallography (SX) experiment at a synchrotron radiation source, the popularity of this approach has continued to grow showing that third-generation synchrotrons can be viable alternatives to scarce X-ray free-electron laser sources. Synchrotron radiation flux may be increased ∼100 times by a moderate increase in the bandwidth ('pink beam' conditions) at some cost to data analysis complexity. Here, we report the first high-viscosity injector-based pink-beam SX experiments. The structures of proteinase K (PK) and A 2A adenosine receptor (A 2A AR) were determined to resolutions of 1.8 and 4.2Å using 4 and 24 consecutive 100ps X-ray pulse exposures, respectively. Strong PK data were processed using existing Laue approaches, while weaker A 2A AR data required an alternative data-processing strategy. This demonstration of the feasibility presents new opportunities for time-resolved experiments with microcrystals to study structural changes in real time at pink-beam synchrotron beamlines worldwide.

Original languageEnglish
Pages (from-to)412-425
Number of pages14
JournalIUCrJ
Volume6
DOIs
Publication statusPublished - Jan 1 2019

Keywords

  • X-ray crystallography
  • injector-based serial crystallography
  • membrane proteins
  • pink-beam serial crystallography
  • protein structures
  • structural biology
  • structure determination
  • third-generation synchrotrons

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry
  • Materials Science(all)
  • Condensed Matter Physics

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  • Cite this

    Martin-Garcia, J. M., Zhu, L., Mendez, D., Lee, M. Y., Chun, E., Li, C., Hu, H., Subramanian, G., Kissick, D., Ogata, C., Henning, R., Ishchenko, A., Dobson, Z., Zhang, S., Weierstall, U., Spence, J. C. H., Fromme, P., Zatsepin, N. A., Fischetti, R. F., ... Liu, W. (2019). High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source. IUCrJ, 6, 412-425. https://doi.org/10.1107/S205225251900263X