Identification of a Na+-Binding Site near the Oxygen-Evolving Complex of Spinach Photosystem II

Jimin Wang; Joshua M. Perez-Cruet; Hao Li Huang; Krystle Reiss; Christopher J. Gisriel; Gourab Banerjee; Divya Kaur; Ipsita Ghosh; Alisha Dziarski; M. R. Gunner; Victor S. Batista; Gary W. Brudvig

doi:10.1021/acs.biochem.0c00303

Identification of a Na⁺-Binding Site near the Oxygen-Evolving Complex of Spinach Photosystem II

Jimin Wang, Joshua M. Perez-Cruet, Hao Li Huang, Krystle Reiss, Christopher J. Gisriel, Gourab Banerjee, Divya Kaur, Ipsita Ghosh, Alisha Dziarski, M. R. Gunner, Victor S. Batista, Gary W. Brudvig

Yale University

Research output: Contribution to journal › Article › peer-review

Abstract

The oxygen-evolving complex (OEC) of photosystem II (PSII) is an oxomanganese cluster composed of four redox-active Mn ions and one redox-inactive Ca2+ ion, with two nearby bound Cl- ions. Sodium is a common counterion of both chloride and hydroxide anions, and a sodium-specific binding site has not been identified near the OEC. Here, we find that the oxygen-evolution activity of spinach PSII increases with Na+ concentration, particularly at high pH. A Na+-specific binding site next to the OEC, becomes available after deprotonation of the D1-H337 amino acid residue, is suggested by the analysis of two recently published PSII cryo-electron microscopy maps in combination with quantum mechanical calculations and multiconformation continuum electrostatics simulations.

Original language	English
Pages (from-to)	2823-2831
Number of pages	9
Journal	Biochemistry
Volume	59
Issue number	30
DOIs	https://doi.org/10.1021/acs.biochem.0c00303
Publication status	Published - Aug 4 2020

ASJC Scopus subject areas

Biochemistry

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Identification of a Na⁺-Binding Site near the Oxygen-Evolving Complex of Spinach Photosystem II. / Wang, Jimin; Perez-Cruet, Joshua M.; Huang, Hao Li; Reiss, Krystle; Gisriel, Christopher J.; Banerjee, Gourab; Kaur, Divya; Ghosh, Ipsita; Dziarski, Alisha; Gunner, M. R.; Batista, Victor S.; Brudvig, Gary W.

In: Biochemistry, Vol. 59, No. 30, 04.08.2020, p. 2823-2831.

Research output: Contribution to journal › Article › peer-review

Wang, Jimin ; Perez-Cruet, Joshua M. ; Huang, Hao Li ; Reiss, Krystle ; Gisriel, Christopher J. ; Banerjee, Gourab ; Kaur, Divya ; Ghosh, Ipsita ; Dziarski, Alisha ; Gunner, M. R. ; Batista, Victor S. ; Brudvig, Gary W. / Identification of a Na⁺-Binding Site near the Oxygen-Evolving Complex of Spinach Photosystem II. In: Biochemistry. 2020 ; Vol. 59, No. 30. pp. 2823-2831.

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title = "Identification of a Na+-Binding Site near the Oxygen-Evolving Complex of Spinach Photosystem II",

abstract = "The oxygen-evolving complex (OEC) of photosystem II (PSII) is an oxomanganese cluster composed of four redox-active Mn ions and one redox-inactive Ca2+ ion, with two nearby bound Cl- ions. Sodium is a common counterion of both chloride and hydroxide anions, and a sodium-specific binding site has not been identified near the OEC. Here, we find that the oxygen-evolution activity of spinach PSII increases with Na+ concentration, particularly at high pH. A Na+-specific binding site next to the OEC, becomes available after deprotonation of the D1-H337 amino acid residue, is suggested by the analysis of two recently published PSII cryo-electron microscopy maps in combination with quantum mechanical calculations and multiconformation continuum electrostatics simulations. ",

author = "Jimin Wang and Perez-Cruet, {Joshua M.} and Huang, {Hao Li} and Krystle Reiss and Gisriel, {Christopher J.} and Gourab Banerjee and Divya Kaur and Ipsita Ghosh and Alisha Dziarski and Gunner, {M. R.} and Batista, {Victor S.} and Brudvig, {Gary W.}",

note = "Funding Information: The authors acknowledge support by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences, and Biosciences, Photosynthetic Systems; experimental work was funded by Grant DE-FG02-05ER15646 (G.W.B.), and computational work was funded by Grant DE-SC0001423 (M.R.G. and V.S.B.). ",

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doi = "10.1021/acs.biochem.0c00303",

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AU - Wang, Jimin

AU - Perez-Cruet, Joshua M.

AU - Huang, Hao Li

AU - Reiss, Krystle

AU - Gisriel, Christopher J.

AU - Banerjee, Gourab

AU - Kaur, Divya

AU - Ghosh, Ipsita

AU - Dziarski, Alisha

AU - Gunner, M. R.

AU - Batista, Victor S.

AU - Brudvig, Gary W.

N1 - Funding Information: The authors acknowledge support by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences, Division of Chemical Sciences, Geosciences, and Biosciences, Photosynthetic Systems; experimental work was funded by Grant DE-FG02-05ER15646 (G.W.B.), and computational work was funded by Grant DE-SC0001423 (M.R.G. and V.S.B.).

PY - 2020/8/4

Y1 - 2020/8/4

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AB - The oxygen-evolving complex (OEC) of photosystem II (PSII) is an oxomanganese cluster composed of four redox-active Mn ions and one redox-inactive Ca2+ ion, with two nearby bound Cl- ions. Sodium is a common counterion of both chloride and hydroxide anions, and a sodium-specific binding site has not been identified near the OEC. Here, we find that the oxygen-evolution activity of spinach PSII increases with Na+ concentration, particularly at high pH. A Na+-specific binding site next to the OEC, becomes available after deprotonation of the D1-H337 amino acid residue, is suggested by the analysis of two recently published PSII cryo-electron microscopy maps in combination with quantum mechanical calculations and multiconformation continuum electrostatics simulations.

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