Imaging Supramolecular Morphogenesis with Confocal Laser Scanning Microscopy at Elevated Temperatures

Hiroaki Sai, Garrett C. Lau, Adam J. Dannenhoffer, Stacey M. Chin, Luka Dorddević, Luka Dorddević, Samuel I. Stupp, Samuel I. Stupp, Samuel I. Stupp, Samuel I. Stupp, Samuel I. Stupp

Research output: Contribution to journalArticle

Abstract

The morphogenesis of supramolecular assemblies is a highly dynamic process that has only recently been recognized, and our understanding of this phenomenon will require imaging techniques capable of crossing scales. Shape transformations depend both on the complex energy landscapes of supramolecular systems and the kinetically controlled pathways that define their structures and functions. We report here the use of confocal laser scanning microscopy coupled with a custom-designed variable-temperature sample stage that enables in situ observation of such shape changes. The submicrometer resolution of this technique allows for real-time observation of the nanostructures in the native liquid environments in which they transform with thermal energy. We use this technique to study the temperature-dependent morphogenic behavior of peptide amphiphile nanofibers and photocatalytic chromophore amphiphile nanoribbons. The variable-temperature confocal microscopy technique demonstrated in this work can sample a large volume and provides real-time information on thermally induced morphological changes in the solution.

Original languageEnglish
Pages (from-to)4234-4241
Number of pages8
JournalNano letters
Volume20
Issue number6
DOIs
Publication statusPublished - Jun 10 2020

Keywords

  • confocal microscopy
  • in situ microscopy
  • nanofibers
  • nanoribbons
  • supramolecular assembly

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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  • Cite this

    Sai, H., Lau, G. C., Dannenhoffer, A. J., Chin, S. M., Dorddević, L., Dorddević, L., Stupp, S. I., Stupp, S. I., Stupp, S. I., Stupp, S. I., & Stupp, S. I. (2020). Imaging Supramolecular Morphogenesis with Confocal Laser Scanning Microscopy at Elevated Temperatures. Nano letters, 20(6), 4234-4241. https://doi.org/10.1021/acs.nanolett.0c00662