Improved depth resolution in video-rate line-scanning multiphoton microscopy using temporal focusing

Eran Tal, Dan Oron, Yaron Silberberg

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

By introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain video-rate images with depth resolution similar to point-by-point scanning multiphoton microscopy while mechanically scanning in only one dimension. This is achieved by temporal focusing of the illumination pulse: The pulsed excitation field is compressed as it propagates through the sample, reaching its shortest duration (and highest peak intensity) at the focal plane before stretching again beyond it. This method is applied to produce, in a simple and scalable setup, video-rate two-photon excitation fluorescence images of Drosophila egg chambers with nearly 100,000 effective pixels and 1.5 μm depth resolution.

Original languageEnglish
Pages (from-to)1686-1688
Number of pages3
JournalOptics Letters
Volume30
Issue number13
DOIs
Publication statusPublished - Jul 1 2005

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ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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